Expression Of CDH13 And EZH2 In Ovarian Cancer And Clinical Significance

Objective To examine the expression and methylation levels of CDH13 and evaluate its correlation with EZH2 and its clinical significance in epithelial ovarian cancer.Methods(1)The expression of CDH13 and EZH2 protein in ovarian tissues were detected by immunohistochemisty,including in normal ovary tissues and ovarian cancer tissues.and then the association between EZH2 and CDH13 was analyzed by using SPSS.(2)The expression of CDH13 and EZH2 protein in ovarian cancer cells were detected by Western Blot.(3)The DNA methylation level of CDH13 in ovarian cancer cells was measured by pyrosequencing,and then the association between EZH2 and CDH13 was analyzed by using SPSS.Results(1)The expression of EZH2 protein was significantly increased in epithelial ovarian cancer tissues(p<0.05),and the expression of EZH2 was correlated with clinical stages and lymphatic metastasis in epithelial ovarian cancer(p<0.05),but had no significant correlation with age,tumor differentiation and menopause of ovarian cancer patients(p>0.05).(2)The expression of CDH13 protein was significantly decreased in epithelial ovarian cancer tissues(p<0.05),and the expression of CDH13 was correlated with clinical stages and lymphatic metastasis in epithelial ovarian cancer(p<0.05),but had no significant correlation with age,tumor differentiation and menopause of ovarian cancer patients(p>0.05).(3)CDH13 expression was inversely correlated with EZH2 expression in epithelial ovarian cancer(p<0.05).(4)The expression of EZH2 protein was significantly decreased in SKOV3 cell lines(p<0.05),and there is no significant difference in EZH2 expression among the other three cell groups(p>0.05).The expression of CDH13 protein was significantly increased in SKOV3 cells(p<0.05),and there is no significant difference in CDH13 expression among the other three cell groups(p>0.05).(5)The methylation level of CDH13 was significantly decreased in ES2 cells(p<0.05),and there is no significant difference in CDH13 methylation level among the other three cell groups(p>0.05).(6)The methylation level of CDH13 was irrelevant associated with the expression of CDH13 protein(p>0.05).Conclusion The expression of CDH13 is negatively associated with EZH2 in epithelial ovarian cancer.But EZH2 may regulate the expression of CDH13 gene via a non-DNA-methylation-dependent pathway.