Relationship Between CD163+M2 Type Tumor-associated Macrophages And Clinicopathological Characteristics In Microenvironment Of Breast Cancer

Objective:To investigate the relationship between the expression of CD163+M2 type tumor associated macrophage(TAM)and the microvascular density(MVD)in the tumor microenvironment and the clinicopathological characteristics of invasive ductal carcinoma of breast.Methods:The En Vision two-step immunohistochemical method was used to detect the expressions of CD163 and CD31 in the stroma of 138 cases in breast invasive ductal carcinoma and 35 cases of adjacent normal tissues.Results:The high expression rate of CD163+M2 type TAM in the microenvironment of invasive ductal carcinoma was 61.59%,which was significantly higher than that in the adjacent normal tissues(14.29%),and the difference was statistically significant(P<0.05).The high expression rate of CD163+M2 type TAM in grade III was 80.85%,which was significantly higher than that in grade I(33.33%)and grade II(52.27%)(P<0.05).The expression rate of CD163+M2 type TAM in cases of Ki-67 proliferation index ≥20% was 71.83%,which was significantly higher than Ki-67 proliferation index<20%(50.75%)(P<0.05).The high expression rate of CD163+M2 type TAM in cases of lymph node metastasis was 74.24% and significantly higher than without lymph node metastasis cases(50.00%)of invasive ductal carcinoma(P<0.05).The high expression rates of CD163+M2 type TAM in Luminal B-like HER-2 negative type(65.62%),Luminal B-like HER-2 positive type(65.38%),HER2 positive type(81.82%)and triple negative type(58.82%)were significantly higher than that of Luminal A type(37.50%),respectively,and the HER-2 positive type was clearly observed as a higher expression rate than other moleculae types(P<0.05)at the same time.Also,there was no significant difference between the expression of CD163+M2type TAM and the patients’ age,menstrual status,TNM stage,tumor size,recurrence,distant metastasis and death(P>0.05),respectively.To compare invasive ductal carcinoma with adjacent normal tissue,the CD31 labeled MVD was higher in invasive ductal carcinoma cases(P<0.05).The high rate of MVD with tumor diameter > 5cm was 66.67%,which was significantly higher than cases with tumor diameter >2 cm and ≤5cm(53.73%)and tumor diameter ≤2.0cm(27.78%),respectively.the CD31 labeled MVD rate in Ki-67 proliferation index ≥20% group was 61.64%,which was significantly higher than Ki-67 proliferation index < 20%(40.00%)(P<0.05).The high rate of CD31 labeled MVD in Luminal B-like HER-2 negative(65.62%),Luminal B-like HER-2 positive(34.62%),HER2 positive(63.64%)and triple negative(73.53%)breast cancer were higher than that in Luminal A type(16.67%),and the CD31 labeled MVD rate in triple negative breast cancer group was statistically significantly highest(P<0.05)in all molecular groups.Meanwhile,there was no significant difference between the CD31 labeled MVD and the patients’ age,menstrual status,TNM stage,histological grade,lymph node metastasis,recurrent distant metastasis and death(P>0.05).The expression of CD163+M2 type TAM was positively correlated with CD31 labeled MVD(r=0.180,P<0.05).CD163+M2 TAM expression and CD31 labeled MVD were not associated with prognosis in patients with breast invasive ductal carcinoma(P>0.05).Conclusions:The expression of CD163+M2 TAM is up-regulated in the microenvironment of breast invasive ductal carcinoma,and it maybe promote tumor interstitial angiogenesis, tumor proliferation activity and lymph node metastasis,which has potential value in evaluating tumor biological behavior.