Evaluation Of Oxidative Stress In Children With Autism By H₂O₂ And Fe³⁺-ABTS

ObjectiveOxidative stress may be an important factor in the etiology of autism spectrum disorder（ASD）.The imbalance of oxidative stress ability（OSA）and total antioxidant capacity（TAC）in children with ASD may aggravate the abnormal neurobehavior of children with ASD.This study evaluated the oxidative microenvironment in children with ASD by detecting the levels of plasma hydrogen peroxide（H₂O₂）and Fe³⁺-3-ethylbenzothiazolin-6-sulfonic acid(Fe³⁺-ABTS),for the sake of providing new bjectives for the cure and prevention of children with ASD,and explore the possible independent risk factors of ASD.MethodsFrom November 2020 to November 2021,60 children with ASD diagnosed for the first time at the age of 2～15 were randomly selected from the children in the child health department of Shenyang children’s Hospital,and then According to the comprehensive evaluation results of DSM-5,Child Autism Rating Scale（CARS）,social response scale（SRS）,Wechsler Intelligence Scale for children（WISC）and Autism Behavior Checklist（ABC）,they were separated into mild-group,moderate-group and severe-group,with 20 in each group.At the same time,60 typical development（TD）children aged 2～15 who were treated in the child care department during this period were randomly selected.The concentration of plasma H₂O₂and Fe³⁺-ABTS were measured by UV spectrophotometer colorimetry.The serious data is statistically managed by ibmspss 26.Results1.There were significant divergence in maternal abortion history and mode of delivery among typical development children group,mild ASD group,moderate ASD group and severe ASD group（P<0.05）,moreover,there were significant divergence between any two groups（P<0.05）;There was no significant difference in neonatal gestational age,birth weight and allergy history among the four groups（P>0.05）.2.There were significant divergence in H₂O₂level,Fe³⁺-ABTS level and H₂O₂/Fe³⁺-ABTS ratio among typical development children group,mild ASD group,moderate ASD group and severe ASD group（P<0.05）.3.Levels of plasma Fe³⁺-ABTS in the ASD group showed a negative correlation with the degree of disease（r=-0.934,P<0.01）.However,the level of plasma H₂O₂did not correlate with the degree of disease（r=-0.016,P=0.899）.4.There were significant levels of plasma Fe³⁺-ABTS in the ASD group showed a negative correlation with the degree of disease.gence in plasma H₂O₂level,Fe³⁺-ABTS level and H₂O₂/Fe³⁺-ABTS ratio between ASD group and typical development children group under the age of 6 and between ASD group and typical development children group aged 6～15（P<0.05）.5.The factors with statistically significant difference between mild ASD group,moderate ASD group,severe ASD group and typical development children group were analyzed by multi factor and multi classification logistic regression,with typical development children group as the reference category,it was found that:（1）There were significant divergence in plasma Fe³⁺-ABTS level,plasma H₂O₂level and H₂O₂/Fe³⁺-ABTS ratio between children in mild ASD group and children in typical development children group（P<0.05）.（2）There were significant divergence in plasma Fe³⁺-ABTS level and H₂O₂/Fe³⁺-ABTS ratio between children in moderate ASD group and children in typical development children group（P<0.05）.（3）There were significant divergence in plasma H₂O₂level and H₂O₂/Fe³⁺-ABTS ratio between children in severe ASD group and children in typical development children group（P<0.05）.Conclusions1.The level of plasma Fe³⁺-ABTS in ASD children is lower than that in typical developing children,and negatively correlated with the degree of disease.2.The plasma H₂O₂level of ASD children is higher than that of typical developing children,while there was no correlation with the degree of the disease.3.The plasma Fe³⁺-ABTS level of ASD children decreased with age,and there was no significant difference in the distribution of plasma H₂O₂level in different age groups.4.The increase of oxidative stress and the decrease of antioxidant capacity in ASD children.