| Purpose:The purpose of this experiment is to study the in vitro analysis of the antifungal activity of adding different mass fractions of graphene oxide to acrylic soft lining materials.Method:0%,0.25%,0.5%,1%,2% of graphene oxide was added to the acrylic soft lining material,the unadded group was the control group,and the rest were the experimental group.The experiment uses scanning electron microscopy（SEM）and Fourier transform infrared spectroscopy for characterization and detection.The CCK-8 method is used to detect the effect of denture soft lining materials containing different mass fractions of GO on the proliferation and cytotoxicity of mouse fibroblasts L929./Dead cell staining method was used to detect the influence of each group on the cell viability of L929;the film adhesion method was used to detect the antibacterial rate of each group of specimens against Candida albicans,and then the composite material with the best antibacterial effect was selected for 8h ultraviolet light irradiation and 5000 hot and cold cycle treatments to test the long-term antibacterial effect.Result:1.Scanning electron microscope（SEM）resultsGraphene oxide is uniformly dispersed on the acrylic self-curing soft lining material.The surface of the soft lining material of the control group is the smoothest compared to the other groups of samples.The surface of the soft lining material containing 0.25 wt% GO is slightly rough,but there is no obvious particle or agglomeration.The soft lining material containing 0.5 wt% GO Compared with the soft lining material with 0.25 wt% GO,the particle morphology of the surface is more obvious,while the surface particle morphology of the soft lining material with1.0 wt% GO is more obvious,and there is obvious agglomeration.The soft lining material with 2.0 wt% GO Wrinkles and pits appear on the surface of the lining material.2.Fourier transform infrared spectroscopy analysis resultsFrom the analysis of infrared spectroscopy,it can be seen that the five samples are in the soft lining material and GO functional groups such as-CH2（2958cm-1）,C=O（1719cm-1）,CH（1449cm-1）,COC（1179cm-1）and CO（There are characteristic peaks at 1072cm-1）,and there is no obvious difference in the five groups of waveforms.3.CCK-8 test resultsIn the same time treatment of soft denture lining materials containing different mass fractions of GO,the five kinds of material extracts have no inhibitory effect on cell proliferation.4.Live and dead cell staining resultsObserved by fluorescence microscope,live cells are marked as green and dead cells are marked as red.The survival rates of L929 cells in the blank control group,0wt %,0.25 wt%,0.5 wt%,1.0wt %,and 2.0 wt% groups are all at 98.% Above,there was no significant difference between the groups（P>0.05）.5.Antibacterial rate test resultsBy comparing the antibacterial effects of Candida albicans in each group of samples,it can be clearly found that the control group has the most colonies and dense,while the experimental group has relatively few and sparse colonies.The calculation results showed that the number of Candida albicans colonies in the experimental group was significantly reduced（P <0.01）.The 1 wt% group has an antibacterial rate of 93.04% against Candida albicans,which has an antibacterial effect;the 2.0 wt% group has an antibacterial rate of 99.41% against Candida albicans,which has a strong antibacterial effect.6.Test result of antibacterial rate after agingThe composite material with the best antibacterial effect group（2.0 wt% group）was selected and subjected to 8h ultraviolet light irradiation and 5000 times of heat and cold cycle treatment,and the long-term antibacterial effect was tested.The bacteria still showed good antibacterial properties. |
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