Research On The Application Of Nucleic Acid Point-of-care Testing Technology In The Detection Of Important Viral Infectious Diseases

Viral infectious diseases have always been a serious threat to the lives and health of humans and animals.With the development of science and technology,transportation has become more convenient,and the transmission of viruses has become diverse and the speed of transmission has also increased rapidly.Therefore,rapid detection and accurate identification of pathogens is a prerequisite for effective response to viral infectious diseases,and it also provides guidance for related treatments.At present,the initial diagnosis mainly relies on laboratory testing.Traditional laboratory testing methods rely on various large-scale precision instruments and professional operators,which are time-consuming and costly.my country is a populous country.The relative concentration of the population has resulted in the relative concentration of treatment resources.For county-level cities,medical testing conditions are relatively sufficient,and laboratories have complete testing conditions.The virus can be detected and blocked in the early stages of transmission.spread.However,in the rural areas,the land is large and the population is sparse,the treatment resources are relatively scattered,and the experimental hardware and software conditions in the rural medical and health service facilities are relatively scarce.And with the development of road traffic,the flow of people between urban and rural areas has become more frequent.Therefore,when the virus begins to spread in rural areas,it is easy to cause a large-scale spread of the epidemic.Therefore,in order to meet the needs of rapid detection under simple conditions,on-site rapid detection technology（point-of-care testing,POCT）came into being.The current POCT nucleic acid detection methods include loop-mediated isothermal nucleic acid amplification technology（Loop-Mediated Isothermal Amplification,LAMP）Recombinase polymerase isothermal nucleic acid amplification technology（Recombinase aided amplification,RAA）,multiple real-time fluorescent quantitative PCR technology,etc.This study established the ASFV-RAA-LFD detection method for African swine fever virus,which can be used in A detectable nucleic acid amplification level is reached in 10 minutes at 37℃multiple real-time fluorescent quantitative PCR has been established for influenza A virus,influenza B virus,parainfluenza virus,human metapneumovirus,human rhinovirus and 2019 n Co V method.