Study On The Correlation Between Chronic Spontaneous Urticaria And Intestinal Microbe

Research background: Chronic Spontaneous urticaria（CSU）is a common allergic skin disease,it is widely believed that the mast cells and basophils activation and degranulation is the core mechanism of the disease.More and more scholars put forward Autoimmunity and autoallergy theory can lead to the onset of the CSU,including autoimmune theory more recognized.In addition,the blood coagulation cascade is also thought to participate in the pathogenesis of CSU.Intestinal tract is the largest immune organ of the human body,and studies have found that the imbalance of intestinal flora is closely related to a variety of diseases.A small number of studies have shown that the intestinal flora of CSU patients is different from that of healthy people,and the incidence of CSU may be related to the imbalance of intestinal flora The composition and structure of CSU are different from that of healthy people.There may be specific intestinal biomarkers in CSU,and the metabolites of intestinal flora are related to the pathogenesis of CSU.The purpose of this study was to find the difference in the composition of intestinal flora between patients with chronic spontaneous urticaria and healthy people,to clarify the characteristics of intestinal flora in patients with CSU,to find the possible pathogenesis of CSU,and to provide new ideas for the treatment of CSU.Research Objective: To detect the diversity and composition of intestinal flora in patients with chronic spontaneous urticaria and healthy people by 16 S r RNA sequencing technology.To preliminarily explore the composition and structural characteristics of intestinal flora in patients with chronic spontaneous urticaria,discover possible disease biomarkers,and provide a theoretical basis for the pathogenesis of chronic spontaneous urticaria and new treatment methods.Research Methods: From June 2021 to August 2021,12 patients with chronic spontaneous urticaria（group P）and 12 healthy controls（group N）who are treated in the Department of Dermatology of the First Affiliated Hospital of Anhui Medical University were selected,and the subjects were collected.The fecal samples of the patients are subjected to DNA extraction,PCR amplification of target fragments,library construction,and computer sequencing.After sequencing,species composition analysis,flora diversity analysis,differential species analysis,LEf Se analysis,and PICRUSt analysis are performed.information analysis,etc.Research Results:1.We detect a total of 18693 ASVs,including 8436 ASVs unique to chronic urticaria patients,7836 ASVs unique to healthy individuals,and a total of 2511 ASVs in both groups.2.There is no significant difference in α diversity between the two groups,but the difference in β diversity is statistically significant（P=0.012）,suggesting that the gut microbiota structure between the CSU group and the healthy control group is different.At the phylum level,Proteobacteria and TM7 are significantly increased in the CSU group,and the difference is statistically significant（P<0.05）.At the class level,Alphaproteobacteria,Gammaproteobacteria,TM7-3,and unclassifed_Proteobacteria in the CSU group increased significantly（P<0.05）;At the order level,Rhizobiales,Enterobacteriales,Actinomycetales and unclassifed_proteobacteria in the CSU group increased significantly（P<0.05）;At the family level,Leuconostocaceae,Enterobacteriaceae,unclassifed_Proteobacteria in the CSU group increased significantly（P<0.05）,Ruminococcaceae,Prevotellaceae and Bacillaceae significantly increased in healthy control group（P<0.05）;At the genus level,unclassified_Ruminococcaceae,Prevotellaceae_Prevotella,Faecalibacterium are significantly increased in the healthy control group,and Weissella,unclassified_Peptostreptococcaceae,unclassified_Enterobacteriaceae were significantly increased in the CSU group,Xenorhabdus,Peptostreptococcaceae_<sub>Clostridium,02d06,Allobaculum,unclassified_Proteobacteria increase significantly.3.LEf Se analysis indicates 9 possible biomarkers of CSU,Gammaproteobacteria,Enterobacteriaceae,Enterobacteriales,Proteobacteria,Ruminococcaceae,Bacillaceae,Prevotellaceae,Prevotella.4.PICRUSt function prediction shows that the abundance of ko05322 SLE related metabolic pathways increased significantly in the CSU group（P=0.036）.Conclusion: The results of this study showed that there are no significant differences in the diversity and richness of intestinal flora between CSU patients and healthy controls,but significant differences in the composition and structure of intestinal flora between the two groups.Proteobacteria and Enterobacteriaceae were significantly increased in CSU group,while Prevotella was significantly increased in healthy control group.Nine possible intestinal microbial markers of CSU are found.The abundance of ko05322 systemic lupus erythematosus related metabolic pathways is significantly increased in the CSU group,suggesting that CSU may be associated with the pathogenesis of systemic lupus erythematosus.