Correlation Of Vaginal Microecological Indicators And Cytokines With HPV Infection In LSIL Patients

Objective It has been established that persistent infection with high-risk human papillomavirus（HR-HPV）is the main cause of cervical cancer and its precancerous lesions..If HR-HPV infection persists,approximately 10%of LSIL will progress to HSIL within two years,and approximately 20%of HSIL may progress to invasive carcinoma of the cervix within 10 years.Therefore,it is particularly important to take active intervention measures in the early stage of cervical lesions to prevent cervical lesions and even cervical cancer.Of this study was to analyze LSIL patients vaginal microbial metabolites and enzyme（vaginal PH,vaginal cleanness degree,concentration of hydrogen peroxide（H₂O₂）,saliva acid glycosides enzyme concentration,concentration of leukocyte esterase）and cytokines（vaginal levels of interleukin-2）and the relationship between HPV infection,to adjust the vaginal microecological,reduce the risk of HPV infection,So as to provide theoretical basis for early prevention and prevention of cervical lesions and cervical cancer.Methods A total of 255 patients who were diagnosed with LSIL by biopsy under colposcopy in the department of Gynecology of Hefei Second People’s Hospital from June 2020 to December 2020 were selected（inclusion criteria:（1）age 18-65 years old;（2）Sexual life≥3 years;（3）No recent treatment for vaginitis and cervicitis;（4）Have no sex within 48 hours;（5）Non-menstruation,non-pregnancy and puerperium;（6）No vaginal bleeding or damage）.After investigation and analysis of demographic characteristics and behavioral factors,255 patients were divided into 3 groups according to HPV infection and typing:Vaginal secretion test and vaginal interleukin-2 test were performed in the HPV negative group,low-risk HPV（LR-HPV）infection group and high-risk HPV（HR-HPV）infection group,and the results were statistically analyzed.To investigate the relationship between vaginal microbial metabolites,enzymes and cytokines and HPV infection in LSIL patients.Results 1.Compared with LR-HPV infection group and HPV negative group,HR-HPV infection group had significantly higher smoking rate,younger than 20 years of age at first sexual intercourse,and more than two sexual partners,with statistically significant differences（P<0.05）.There was no significant difference in body mass index among the three groups（P>0.05）.2.The proportion of vaginal PH>4.5 in LR-HPV infection group was higher than that in HPV negative group,and the difference was statistically significant（χ~2=5.446,P<0.05）.There was no significant difference between the two groups in cleanliness degreeⅢtoⅣand the positive rates of hydrogen peroxide,leucocyte esterase and sialidase（P>0.05）.3.Compared with HPV negative group and LR-HPV infection group,hr-HPV infection group had higher vaginal PH>4.5,vaginal cleanlinessⅢtoⅣdegree,positive rates of hydrogen peroxide,leucoesterase and sialidase,with statistical significance（P<0.05）.4.There was no significant difference in the content of interleukin-2 in the HPV negative group compared with the LR-HPV infected group（P=0.211）.The content of interleukin-2 in the vagina of LR-HPV infection group was higher than that of HR-HPV infection group,and the difference was statistically significant（P<0.05）.The vaginal interleukin-2 content in HPV negative group was higher than that in HR-HPV infection group,and the difference was statistically significant（P<0.05）.Conclusions 1.Vaginal microecological imbalance may increase the risk of HPV infection.2.Increased local expression of interleukin-2 in the vagina may reduce the risk of HPV infection.3.By regulating vaginal microecology and reducing the risk of HPV infection,early prevention and blocking of cervical lesions and cervical cancer may be achieved.