Research Of LncRNA CACNA1C-AS2 Regulates PSMD4 On The Proliferation And Invasion Of Esophageal Cancer Cells

Background: As one of the common malignant tumors of the digestive system,esophageal cancer lacks typical symptoms in the early stage.It is prone to metastasis,resulting in high mortality.In recent years,there are more and more studies on the role of Lnc RNA in tumors.So far,there is no report on CACNA1C-AS2 in esophageal cancer.Moreover,the study of PSMD4 in other tumors has been reported,but the research is rare in the esophageal cancer.Therefore,the purpose is to explore the role of CACNA1C-AS2 in esophageal cancer and the effects of CACNA1C-AS2 on the proliferation,migration and invasion abilities and epithelial-mesenchymal transformation（EMT）of esophageal cancer cells by regulating PSMD4.The aim is to provide theoretical evidences for diagnosis and treatment of esophageal cancer.Objective: To investigate the role of CACNA1C-AS2 in esophageal cancer and whether CACNA1C-AS2 affects the proliferation,migration and invasion abilities and EMT of esophageal cancer cells by regulating PSMD4.Methods: The expression of CACNA1C-AS2 in esophageal cancer tissues by q RT-PCR and TCGA database.Meanwhile,the expression of CACNA1C-AS2 were detected in normal esophageal epithelial cells and esophageal carcinoma cell lines.EC-9706 and Eca-109 cellswere used as the objects of study.The efficiency of transfection was determined by q RT-PCR after knocking down and over-expressing CACNA1C-AS2.MTT assay,Transwell assay,flow cytometry and wound healing assay were used to analyze the impact of CACNA1C-AS2 on the biological traits of esophageal cancer cells.Western blot was used to detect the effect of CACNA1C-AS2 on the expression of EMT related protein.q RT-PCR analyzed the expression level of PSMD4 in esophageal carcinoma tissues and cells and the efficiency of transfection.The proliferation,migration and invasion abilities and apoptosis rate of esophageal cancercells were tested after over-expression and knocking down PSMD4.The effects of PSMD4 on the expression of EMT related proteins were detected by Western blot.q RT-PCR and Western blot were used to detect the regulation of CANCA1C-AS2 on PSMD4 expression.The proliferation,migration and invasion abilities and the expression of EMT related proteins of esophageal cancer cells were observed after co-transfection of CACNA1C-AS2 and PSMD4,so as to explore whether CACNA1C-AS2 could affect the occurrence and progression of esophageal cancer by regulating PSMD4.Results: The expression of CACNA1C-AS2 were low in both esophageal cancer tissues and cells.Up-regulated CACNA1C-AS2 decreased the proliferation,migration and invasion abilities,increased the apoptosis rate,and reduced the expression of EMT related protein in esophageal cancer cells.On the contrary,the results were adverse while silencing CACNA1C-AS2.The expressions of PSMD4 were high in esophageal cancer tissues and cells.Knocking down PSMD4 repressed the proliferation,migration and invasion abilities,raised the rate of apoptosis,decreased the expression of EMT related protein in esophageal cancer cells.The results were opposite after PSMD4 was high expression.Over-expression of PSMD4 could partially neutralize the effects of up-regulation of CACNA1C-AS2,which inhibited the proliferation,migration and invasion abilities and suppressed the expression of EMT related proteins of esophageal cancer cells.While silencing PSMD4 could partially neutralize the effects of knocking down CACNA1C-AS2,which promoted the proliferation,migration and invasion abilities,enhanced the expression of EMT related proteins of esophageal cancer cells.Conclusion: CACNA1C-AS2 was low expression in esophageal cancer.CACNA1C-AS2 not only repressed the proliferation,migration and invasion abilities and raised the apoptosis rate,but also suppressed EMT of esophageal cancer cells.PSMD4 was high expression in esophageal cancer.PSMD4 enhanced the proliferation,migration and invasion abilities and mitigated the apoptosis rate of esophageal cancer cells,promoted the EMT.CACNA1C-AS2 exerted a tumor inhibitory role in esophageal cancer cells by down regulating PSMD4.