Study On The Effect And Mechanism Of Pogostone On Human Hepatocellular Carcinoma HepG2 Cells Based On Network Pharmacology

Objective: As the main component in the volatile oil of Pogostemon cablin,Pogostone has antibacterial,antiviral and anti-inflammatory effects.In recent years,it has been found that Pogostone has a significant inhibitory effect on tumour cells,such as colorectal cancer HCT116 cells and gallbladder cancer SGC-996 cells.As a high incidence rate and high mortality rate,the effect of Pogostone on inhibiting hepatocellular carcinoma is not clear.Therefore,this paper elucidates the effect and mechanism of Pogostone on liver cancer through cell experiment and network pharmacology.Methods:1.Through cell experiment,the effect of Pogostone on HepG2 cells was discussed.CCK-8 experiment and colony formation experiment were used to explore the effect of Pogostone on HepG2 cell proliferation.Hoechst 33258 staining experiment and flow cytometry were used to detect the effect of Pogostone on HepG2 cell apoptosis and cycle,The effects of Pogostone on the migration and invasion of HepG2 cells were detected by scratch test and Transwell chamber test,and the expression of related proteins was detected by Western blot.2.The target and mechanism of Pogostone on hepatocellular carcinoma were discussed through network pharmacology.The common drug disease targets were obtained through OMIM,Drugbank,Genecards and Pharm Mapper databases.The network diagram of "disease-target-component" interaction was drawn by Cytoscape software,and the PPI network of protein interaction was constructed by STRING database.Topology analysis was carried out through networkanalyzer tool,and the function enrichment analysis of key target genes go and KEGG was carried out by Bioconductor bioinformatics software package based on R software.Then,the relationship between Pogostone and core target and pathway was verified by molecular docking and Western blot.Results:1.The results of CCK-8 experiment and colony formation experiment showed that Pogostone could significantly inhibit the proliferation of HepG2 cells.Hoechst 33258 staining experiment and flow cytometry showed that Pogostone could induce the apoptosis of HepG2 cells and block its cycle in G2/M phase.The results of scratch test and Transwell chamber test showed that Pogostone could inhibit the migration and invasion of HepG2 cells.The related proteins were detected by Western blot to further verify the above findings.2.The results of network pharmacology showed that there were 52 common targets between Pogostone and hepatocellular carcinoma.The core targets were screened through PPI network.The targets such as HARS,AKT1 and ALB may be the core targets of Pogostone in inhibiting hepatocellular carcinoma.After R software,GO results showed that the intersection gene set was enriched to 1345 biological process pathways,27 cell component expression processes and 83 processes related to molecular function.KEGG results showed that PI3K-Akt signaling pathway may be the most important pathway for Pogostone to inhibit hepatocellular carcinoma.Molecular docking results showed that Pogostone had a good coincidence with the core target AKT1.Western blot showed that Pogostone could inhibit the expression of p-PI3 K and p-Akt in HepG2 cells.Conclusions:1.Pogostone can inhibit the proliferation and induce apoptosis of hepatocellular carcinoma HepG2 cells,block its cycle in G2/M phase,and inhibit its migration and apoptosis.2.The results of network pharmacology show that the inhibitory effect of Pogostone on hepatocellular carcinoma may be related to PI3K-Akt signaling pathway.