The Study Of Intermolecular Interactions Using A Combination Of Physical And Chemical Methods

With the development of science and technology,the integration of theories and technologies in many disciplines has been developed and improved.And the physics,chemistry,biology,and pharmacology have become increasingly connected.Nowadays,the research of a single discipline can no longer meet the rapid development of science and technology.Related interdisciplinary research has gradually been valued by scientific researchers and has become a cutting-edge subject in the scientific field.Among them,the research method of combining physics and chemistry has always been one of the magic weapons of scientific researchers.Physical and chemical methods of ultrasonic technology,biofilm technology,and fluorescence spectroscopy have all become typical and hot research topics that are interdisciplinary and multidisciplinary.In this paper,three research topics,namely the study of the degradation of sewage,the interaction of lycium barbarum polysaccharides(LBP)and lipid monolayers,and the fluorescence quenching effect of LBP on Bovine serum albumin(BSA),are investigated separately to explore the characteristics and advantages of the three technologies,And then find the advantages of the three technologies combined to design experiments to explore.The specific experimental content and results are as follows:(1)Study on degradation of rhodamine B(RB)by ultrasonic radiation and mechanical stirring.In this study,taking RB as the research object,the effects of ultrasonic radiation and mechanical stirring on the degradation of wastewater were systematically studied.The experimental results show that the best experimental conditions for the degradation of RB are:the ultrasonic frequency is 40 kHz,the reaction vessel is a three-necked flask,the initial concentration of the RB solution is 10 mg/L,the ultrasonic irradiation time is 60 min,and the stirring The speed is 700 rpm.Under the optimal conditions,the best degradation rate of RB can reach 98%in one hour.This method can decolorize and degrade dye wastewater such as RB,and the effect of the method of combining ultrasonic radiation and mechanical stirring can achieve the same good degradation effect as the currently popular method of radiating nanoparticles.(2)Study on the interaction between LBP and lipid membrane.The interactions of LBP with DLPC and DSPC neutral lipid monolayers were studied by π-A isotherm,π-t isotherm and compression modulus.From the results obtained,it can be concluded that the forces of LBP interacting with the two lipid monolayers are Van der Waals forces.By studying the π-A isotherm,it can be known that when the LBP solution is added to the subphase,the initial average molecular area moves in an increasing direction,and the lipid monolayer is continuously expanding.By studying the π-t isotherm,we can know that LBP molecules are continuously adsorbed on the monolayer membrane over time,and the greater the concentration of LBP,the more significant the adsorption effect.By studying the Cs-1-π curve,it can be known that as the concentration of LBP increases,the elasticity and compressibility of the monolayer film also decrease.Through the comparison of DLPC and DSPC,it is found that due to the chain length,the adsorption of DSPC monolayer is better than that of DLPC.(3)Study on the quenching effect of LBP on BSA fluorescence.This study determined that the quenching mechanism of LBP on BSA is a combination of static quenching and dynamic quenching.The test results showed that BSA and LBP combined to form a low-fluorescence complex,and energy transfer occurred between them.It can be known by calculation that the energy transfer efficiency and binding distance between BSA and LBP are within a reasonable range.The surface conformation of BSA and LBP was observed by atomic force microscope to characterize the combination of the BSA and LBP.Through the above research,we can have a comprehensive understanding of the role of ultrasound,the characteristics of LB film,and fluorescence technology.The research focuses on the effects of ultrasound on the fluorescence characteristics of special film materials as the entry point to integrate the three directions of ultrasonic radiation technology,LB film formation method and fluorescence spectroscopy.The overall idea is to use ultrasonic radiation to process the material at different times,then use the LB film instrument to make the processed sample into a two-dimensional film-like structure,and finally use fluorescence spectroscopy to characterize the changes in the fluorescence characteristics of the film structure.