Preparation Of Smart Composite Nanomaterials And Investigation In Loading Anticancer Drugs

Intelligent hydrogel microspheres show good biocompatibility and could change their physical-chemical properties in response to environmental stimuli,such as temperature,pH,light and magnetic field.As a drug delivery system,the development of intelligent hydrogel nanoparticles that could transport and deliver chemdrugs selectively to the tumor area is a recent research direction for improving therapeutic efficacy effectively and avoiding systemic side effects,such as renal toxicity,inflammation,nausea,etc.In this paper,silica spheres/smart polymer and luminescent EuW10 were integrated to form core-shell composite particles.The drug delivery of nanocomposite carrier,luminescence and vitro cytoyoxicity were investigated.1.The temperature-sensitive polymer PNIPAM and fluorescent EuW10 were grafted on the surface of SiO2 nanospheres,and SiO2/PNIPAM/EuW10 complexes with core-shell structure were obtained.The final SiO2/PNIPAM/EuW10 complex as drug delivery system shows the response to temperature stimuli.They could be loaded with the anticancer drug doxorubicinhy drochloride(DOX),a commonly used chemotherapeutic drug for cancer therapy,which shows a good release at high temperatures.In cytotoxicity assay,the blank material has good biocompatibility and shows no significant cytotoxic on the HeLa cells in a wide range of concentration,while the DOX-loaded SiO2/PNIPAM/EuW10 composites show obvious inhibitory effect on HeLa cells.2.The temperature/pH dual-responsive polymer P(NIPAM-AAm)was coated on the surface of SiO2 nanospheres to form core-shell SiO2/P(NIPAM-AAm)complex,grafted by a small amount of fluorescent EuW10.SiO2/P(NIPAM-AAm)/EuW10 complexes were prepared.DOX was selected as a model drug to evaluate the loading and controlled release behaviors of the composite.SiO2/P(NIPAM-AAm)/EuW10 nanoparticals show high drug loading capacity and good drug release effect at higher temperature and in PBS with lower pH.In the test of cytotoxicity,the blank material SiO2/P(NIPAM-AAm)/EuW10 shows no significant cytotoxic on the HeLa cells with a wide series of concentration.DOX-loaded SiO2/P(NIPAM-AAm)/EuW10 exhibited higher cytotoxicity than blank material on HeLa cells at a certain concentration,correspondingly.3.The temperature/pH dual-responsive polymer P(DEA-MAA)was coated on the surface of silica nanospheres to form SiO2/P(DEA-MAA)gel complex and then EuW10 fluorescent material was grafted.The fluorescence compound with temperature/pH dual-sensitive properties was prepared,finally.As a drug delivery system,the SiO2/P(DEA-MAA)/EuW10 complex shows a high loading rate and encapsulation efficiency for the anticancer drug DOX.In study of intelligent drug release,composites show the good drug release effect at higher temperature and in PBS with lower pH.In cytotoxicity assay,the blank material SiO2/P(DEA-MAA)/EuW10 nanoparticals show very low toxicity at the tested concentration.However,the DOX-loaded SiO2/P(DEA-MAA)/EuW10 composite materials show significant toxicity on HeLa cells at a certain range of concentration.