| With the rapid development of economy and science and technology,human dietary patterns have gradually changed.The number of people who developed obesity through the intake of high-fat diets has been rising year by year.Obesity has also been accompanied by a series of related adverse reactions and chronic diseases.In recent years,functional ingredients of food have become one of the research focuses of the society to improve obesity and unhealthy body condition caused by high-fat diet.Sonchus arvensis L.has a unique flavor which is a kind of widely distributed wild vegetable in northern China.It is rich in nutrition,such as a variety of polyphenols,flavonoids,including luteolin,cichoric acid and other biologically active components which are anti-obesity and have a regulatory role on glucose metabolism,lipid metabolism.However,it has not yet been reported whether the combined extract of Sonchus arvensis L.has the effect of suppressing obesity.Therefore,this project has a positive effect on the potential nutrition value of Sonchus arvensis L.and its abundant resources.(1)Analysis of total polyphenols and flavonoids in aqueous extract and ethanol extract of Sonchus arvensis L..Ultraviolet spectrophotometry was used to determine the total polyphenols and flavonoids content.Results were calculated by a standard curve.1 mg of Sonchus arvensis L.aqueous and alcohol extracts contains total polyphenols of0.0821±0.0021 mg,0.1391±0.007 mg,respectively.The contents of total flavonoids in the aqueous and alcohol extracts were 0.1928±0.0009 mg and 0.3428±0.0033 mg,respectively.The main components were detected by high performance liquid chromatography,and lucorin,cichoric acid,and chlorogenic acid was the main active substance in the aqueous extract and ethanol extract.The content of luteolin was the highest which was 0.0311±0.0061 in aqueous extracts and 0.0249±0.0058 mg/mg in ethanol;the content of cichoric acid was0.0235±0.0044,0.0198±0.0017 mg/mg,respectively and the contents of chlorogenic acid were 0.0092±.0.0026,0.0053 ± 0.0032 mg/mg,respectively.(2)The 3T3-L1 pre-adipocyte model was used to verify the intervention effect of Sonchus arvensis L.extract on obesity.The results showed that the extract of Sonchus arvensis L.could inhibit the viability of 3T3-L1 preadipocytes,and the degree of inhibition was positively correlated with the extract concentration;it inhibited the lipid droplet accumulation during the differentiation of 3T3-L1 preadipocytes.C57BL/6J mice were induced by high fat diet(HFD)to establish an obese animal model,and to verify the intervention of Sonchus arvensis L.extract on obese mice.The results of the study showed that the extract of Sonchus arvensis L.had a significant inhibitory effect on the increase of body size and weight gain in HFD-induced mice,and it has a certain improving effect on obesity-induced cognitive impairment in obese mice.And it significantly improved the increasing weight of liver and adipose tissue and increasing accumulation of lipid droplets.In summary,the Sonchus arvensis L.extract has a positive regulatory effect on obesity caused by HFD.(3)Research and development of Sonchus arvensis L.-products.According to the effect of the Sonchus arvensis L.extract on anti-obesity,a meal replacement powder based on the Sonchus arvensis L.was selected as the main raw material.The optimal product formula was determined through orthogonal experiment design to ensure that it had unique nutrients,flavor and taste.It was suitable for people who needed to relief obesity.Using a single-factor and orthogonal test to determine the formula of meal replacement powder,the optimum ratio was finally obtained as: 5% xylitol content,40% red bean glutinous rice flour content,8%konjak flour content and the content of Sonchus arvensis L.is 3%.The product has the unique flavor of chicory,moderate bitterness,light green color,even texture,delicate taste,no off flavor and is hard to stratified. |
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