Study On The Interaction Of Ferritin-Iron-Chlorogenic Acid And Its Physicochemical Properties

Ferritin has the function of maintaining the balance of iron metabolism in the body,which can oxidize a large amount of Fe2+,stores it in the form of mineralized nuclei in the cavity of ferritin,and transfers it to the outside of ferritin.At the same time,iron is one of the essential trace elements of the human body.Iron deficiency can cause a series of irondeficiency diseases,but excessive iron can cause toxicity to the body.With strong antioxidant,free radical scavenging,antiviral,antibacterial,and anti-inflammatory effects,chlorogenic acid is a polyphenol widely present in plants,vegetables,and fruits.This paper mainly studies the mechanism of ferritin-Fe3+-chlorogenic acid interaction and its physical and chemical properties.Firstly,the ferritin required for the experiment was successfully prepared by the extraction and puriflcation method of ferritin,and then the ferritin-Fe3+-chlorogenic acid mixture was prepared with different molar ratios.The interaction between ferritin-Fe3+chlorogenic acid was studied by ultraviolet-visible spectroscopy,fluorescence spectroscopy,circular dichroism chromatography,and infrared spectroscopy.This study preliminarily proved that in the ferritin-Fe3+-chlorogenic acid system,chlorogenic acid and Fe3+ formed metal complexes,and there was an interaction between the complex and ferritin,and the interaction was mainly through hydrogen bonding.Secondly,kinetic analysis of ferritin iron oxidation precipitation and reduction release by ultraviolet-visible spectrophotometer found that chlorogenic acid can promote ferritin iron oxidation precipitation and reduction release,and in the process of ferritin iron reduction release,Chlorogenic acid had a strong ability to chelate Fe2+to capture HO·,and can effectively reduce the oxidative damage of free radicals to ferritin.Because iron could induce the polymerization of ferritin,static light scattering was used to perform kinetic analysis,and showed that Fe3+ induced ferritin polymerization,but Fe2+could not directly induce ferritin polymerization.Meanwhile,chlorogenic acid could effectively reduce the degree of Fe3+-induced ferritin polymerization,and could also reduce the degree of polymerization of ferritin itself.Finally,the stability of ferritin in the ferritin-Fe3+-chlorogenic acid complex was analyzed by simulated in vitro digestion.The results showed that the ferritin-Fe3+chlorogenic acid complex could effectively improve the anti-pancreatin stability of ferritin,but the stability against protease had not been significantly improved.The photostability and thermal stability analysis of chlorogenic acid in the ferritin-chlorogenic acid complex by high-performance liquid chromatography showed that ferritin could effectively improve the photostability and thermal stability of chlorogenic acid.In this study,by exploring the mechanism of ferritin-Fe3+-chlorogenic acid interaction and its physical and chemical properties,it was shown that in the ferritin-Fe3+-chlorogenic acid complex,Fe3+ and chlorogenic acid reacted to form a metal complex.The complex interacts with ferritin mainly through hydrogen bonding.At the same time,it was proved that chlorogenic acid could effectively promote the iron oxidation deposition and iron release of ferritin,and could also improve the stability of ferritin.This study provided a new idea for the ferritin-iron interaction of chlorogenic acid active components,and the protein-metalpolyphenol interaction model was built to lay a certain foundation for the interaction of other food components.