Study On Chemical Constituents Of Stems And Leaves Of Alpinia Oxyphylla Miq.and Its Feed Additives

Zingiberaceae(Alpinia oxyphylla Miq)is a perennial herb,which likes shade and blooms twice a year.Alpinia oxyphylla fruit has been listed as a medicine food homologous plant by the Ministry of health.Alpinia oxyphylla fruit has been included in the Chinese Pharmacopoeia(2015 Edition).It has rich pharmacological activities and is widely used in food,health products and other fields.At present,the research on the value utilization of Alpinia oxyphylla fruit has been gradually improved,but the non-traditional medicinal value parts are rarely reported.In this project,the stem and leaf of Alpinia oxyphylla were taken as the research object,and the chemical composition and the value development of additives were studied,so as to provide some scientific reference for the resource utilization of stem and leaf of Alpinia oxyphylla in the future.In this study,different antioxidant experiments in vitro(DPPH,ABTS,FRAP,lipid peroxidation,hydroxyl free radical)were used to screen the different polar extraction segments of the ethanol extract from the stems and leaves of Alpinia oxyphylla.The chemical components of the stems and leaves of Alpinia oxyphylla were isolated,purified and identified by various means,and the antibacterial activities of the monomer compounds were studied.At the same time,the development value of Alpinia oxyphylla stem and leaf as feed additive was studied.The main results are as follows:(1)The results showed that the Et OAc phase of stem and n Bu OH phase of leaf had the best antioxidant activity.DPPH,ABTS,hydroxyl radical scavenging rate and FRAP activity of Alpinia oxyphylla stem were the highest in Et OAc phase and n Bu OH phase of Alpinia oxyphylla leaf,while the inhibition rate of lipid peroxidation was the highest in Et OAc phase of Alpinia oxyphylla stem and leaf.(2)Ten compounds were isolated and identified from the stems and leaves of Alpinia oxyphylla Miq.: kaemferol-4’-methylether(1),β–sitosterol(2),kaempferol-7,4’-dimethyl ether,4 ’-dimethyl ethyl(3),izalpini(4),palmitic acid(5),tectochrysin(6),rhamnocitrin(7),vanillic acid(8),3,3’-di-o-methylellagic acid(9),p-coumaric acid(10).Compounds(7),(9)and(10)were isolated from the stems and leaves of Alpinia oxyphylla for the first time(3)Among the antibacterial activities of compounds,compounds had no obvious inhibitory activity against Staphylococcus aureus at the concentration of 2 mg/m L,but compounds(1),(3)-(6),(8)and(9)had certain antibacterial effect against Escherichia coli,and their minimum inhibitory concentrations were 0.75 mg/m L,1.5mg/m L,0.75 mg/m L,0.5 mg/m L,0.75 mg/m L and 0.75 mg/m L,respectively.(4)A mouse feed AL-1 was designed with stem and leaf of Alpinia oxyphylla and Spirulina platensis as additives.In the nutritional composition of AL-1,the content of crude protein reached 26.65%;the contents of vitamin A,vitamin D and vitamin B12 were higher than the national standard,in which the content of vitamin B12 was 90.5 times higher than the national standard;the contents of trace elements iron and zinc were 328.65 mg/kg and 153.25 mg/kg,respectively.Compared with the SPF group,the weight gain rate of mice in the designed group was 31.25%,which was higher than that in the SPF group.The results showed that the antioxidant activities of the stems and leaves of Alpinia oxyphylla were high,which provided a theoretical basis for the follow-up research of non-traditional medicinal parts of Alpinia oxyphylla.Most of the compounds isolated from Alpinia oxyphylla had certain pharmacological activities,which could be further explored as the possibility of drug research and development.The feasibility of Alpinia oxyphylla stem and leaf as feed additive further indicated its development and utilization value in the aspect of additive.