In Vitro Pharmacodynamic Evaluation Of Oyster Polypeptides And Preparation Of Mask Containing Oyster Polypeptides

Purpose:To determine the anti-aging activity of oyster peptides and combine it with traditional masks to prepare a mask with anti-aging effects.Methods:（1）Human skin fibroblasts（HFF-1）treated with hydrogen peroxide solution（H₂O₂）and different concentrations of oyster polypeptide solution（OPP）were divided into groups,and SA-β-Gal staining kit,MDA kit and SOD kit to detect the anti-aging effects of OPP in different groups;（2）UPLC-Q-TOF/MS combined with multivariate statistical analysis to explore the anti-aging mechanism of OPP（3）Add OPP with anti-aging activity to the traditional mask,and investigate the physical and chemical indicators and sensory of the mask.Results:（1）OPP can enhance the antioxidant capacity of HFF-1 cells to a certain extent,reduce oxidative damage,and delay skin aging.（2）Non-targeted metabolomics analysis found that OPP and aging-related metabolic pathways include glycerophospholipid metabolism,niacin and nicotinamide metabolism,arginine biosynthesis,fatty acid synthesis and decomposition,purine metabolism,etc.（3）The moisture retention,anti-oxidation,oiliness and viscosity of the film under the appropriate formula are the best,and the stability and pH value meet the QB/T 2872-2017 standard.It is a healthy cosmetic skin care product.Conclusion:（1）Oyster polypeptide has significant anti-aging activity.（2）The anti-aging mechanism of OPP may be to change the content of anti-aging active substances and the speed of cell metabolism.（3）Combining OPP with traditional facial masks to develop a mask with anti-aging effects has great development prospects.