Extraction And Invitro Activity Of Extracellular Polysaccharide From Chlorella SP

Polysaccharide is a kind of polymer widely found in the biological world,the current research on Chlorella polysaccharide is more inclined to the research of intracellu Lar polysaccharide,while the research on Chlorella extracellu Lar polysaccharide is less.In this study,the supernatant of Chlorella was selected for the experiment,and the extraction of Chlorella extracellu Lar polysaccharide,component determination,antioxidant index,in vitro simu Lated digestion test,isolated rabbit duodenμm test and in vitro cell test were carried out to provide a theoretical basis for the subsequent This study will provide a theoretical basis for the subsequent industrial development of Chlorella extracellu Lar polysaccharides inhealth care products and cosmetics.The main research contents are as follows.The extracellu Lar polysaccharides obtained from the supernatant of Chlorella were concentrated and purified,and the total sugar content of the extracted extracellu Lar polysaccharides was 8.036 mg/L with a purity of80.000%by p Henol su Lfuric acid method;the extracellu Lar polysaccharides of Chlorella were composed of fourhomogeneous and symmetrical peaks byhigh performance gel permeation chromatograp Hy,and the dispersion coefficients of the polysaccharides in each range were less than 2 with a more uniform distribution;the The molar percentages of D-mannose:glucuronide:glucose:galactose of the extracellu Lar polysaccharide of Chlorella vu Lgaris were48.75:15:11.25:25 byhigh performance liquid chromatograp Hy peaks.The scavenging ability of Chlorella extracellu Lar polysaccharides for DPPH,ABTS~+andhydroxyl radicals was 54.740%,42.400%and 42.980%when the concentration of Chlorella extracellu Lar polysaccharides was 1.00 mg/m L,respectively.The inhibition rates of Chlorella vu Lgaris extracellu Lar polysaccharide against Pseudomonas aeruginosa,Escherichia coli,Salmonella,and Stap Hylococcus aureus were 28.000%,30.000%,12.000%,and 14.000%,respectively,at a concentration of 1.00 mg/m L.In vitro digestion experiments showed that the extracellu Lar polysaccharide of Chlorella vu Lgaris cou Ld be decomposed in gastric juice,and the decomposition effect in intestinal juice was weaker than that in gastric juice.It was found that the extracellu Lar polysaccharide of Chlorella vu Lgarishad a pro-contractile effect on the isolated rabbit duodenμm,and the pro-contractile effect on the isolated rabbit duodenμm reached 177%at the sample concentration of 1 mg/m L,and the samplehad an inhibitory effect on the contraction of the isolated rabbit duodenμm after the addition ofhistamine,carbamide and bariμm chloride pro-contractile drugs,and the sample concentrations were 0.05 mg/m L,0.05 mg/m L and 1.00 mg/m L,respectively.The best inhibition ofhistamine,carbamide and bariμm chloride contraction was observed at concentrations of 0.05 mg/m L,0.05 mg/m L and 1.00mg/m L,respectively.The cell activity assay showed that the extracellu Lar polysaccharide of Chlorella vu Lgarishad a promotional effect on the differentiation and growth of immune cells,and the sampleshad a pro-growth effect on primary mouse splenocytes treated with lipopolysaccharide and knife-bean protein A.The promotional effect was proportional to the concentration;ithad a certain pro-growth effect on abdominal macrop Hages,reduced their NO production and improved p Hagocytic activity;ithad no toxic effect on VERO cells by The besthalf oxidative damage conditions were obtained by setting different concentrations ofhydrogen peroxide and the time of action.0.015%of the action was selected for 1.0h.The resu Lts showed that the samples cou Ld reduce the oxidative damage ofhydrogen peroxide on VERO cells regardless of the successive addition ofhydrogen peroxide.