| In order to solve problems such as bacterial infections,antibiotics came into being.However,with the abuse of antibiotics,bacteria have developed drug resistance,and even super-resistant bacteria have emerged.Because nano-silver has excellent antibacterial ability,and nano-silver is not easy to make bacteria resistant to it,it has become a research hotspot of antibacterial agents.This study uses chemical methods to reduce silver ions to nano-silver,which proves that low-concentration nano-silver can completely kill bacteria,and nano-silver is used as the only photosensitizer to produce a large amount of active oxygen under the synergistic effect of blue light.The concentration required for complete sterilization of nano silver.The main research results of this experiment are as follows:(1)Preparation and characterization results analysis of nano-silver FNP-Ag NC antibacterial drugs.In this study,sodium borohydride was used as a reducing agent;bovine serum albumin and tannic acid were used as complexing agents to construct nano-silver.The prepared nano-silver was characterized by ultraviolet spectrophotometer,transmission electron microscope,dynamic light scattering and Fourier infrared spectroscopy.The maximum characteristic absorption peak of nano-silver measured by ultraviolet spectrophotometer was about 390 nm,which proved the nano-silver’s Exist;Transmission electron microscopy results show that nano-silver is a spherical particle with a diameter of 50±7 nm;the hydrodynamic diameter of nano-silver is 135±2 nm,and PDI<0.3 indicates that nano-silver has good dispersibility;Fourier infrared spectroscopy results It was proved that the reaction between the functional groups of bovine serum albumin and tannic acid occurred,and the samples containing nano-silver showed an absorption peak at 1403 cm-1.(2)Use 72 k J(k J)blue LED lamp to irradiate the synthesized FNP-Ag NC to obtain the B-FNP-Ag NC solution.By detecting the active oxygen content of FNP-Ag NC nano silver before and after illumination,it is proved that the nano silver active oxygen after illumination The content increased from 28621.27±2350.06 to113319.17±2464.33.The FNP-Ag NC and B-FNP-Ag NC solutions were plated and biofilm inhibited experiments.The results of the plated plate experiment showed that the minimum inhibitory concentration of FNP-Ag NC was 3 μg/m L,and the minimum inhibitory concentration of B-FNP-Ag NC was 1 μg/m L,the results of the biofilm inhibition experiment showed that FNP-Ag NC completely inhibited the production of biofilm at a concentration of 3 μg/m L,and B-FNP-Ag NC completely inhibited the production of biofilm at a concentration of 1 μg/m L.Through comparative analysis,we found that the nano-silver solution irradiated by blue light showed higher antibacterial performance and reduced the minimum inhibitory concentration of FNP-Ag NC.The cytotoxicity test was performed on the synthesized nanosilver.The cell viability of the 48-hour FNP-Ag NC treatment group was 90.82%,and the cell viability of the B-FNP-Ag NC treatment group was 92.00%,indicating FNP-Ag NC and B-FNP-Ag NC No obvious toxicity to cells.(3)In order to verify whether the content of 3 μg/m L FNP-Ag NC and 1 μg/m L B-FNP-Ag NC nano silver ointment can achieve the purpose of promoting wound healing at this low concentration,this study established a scald wound model to Analysis of wound healing in different administration groups showed that both the FNP-Ag NC and B-FNP-Ag NC treatment groups basically healed within 15 days,while the control group did not heal completely.Bacteria from the wounds of the different administration groups were collected on the 11 th day.They were cultured on agar plates,and it was observed that there was no bacterial growth in the FNP-Ag NC and B-FNP-Ag NC experimental groups,and bacterial growth in the control group,indicating that the experimental group had achieved the purpose of anti-bacterial infection;cut wound tissues of different administration groups for staining It was observed that the healing effect of the control group was poor,while the FNP-Ag NC and B-FNP-Ag NC groups showed complete epidermal structure,dermal epidermal adhesion,collagen fibers,new microvessels,and epidermal regeneration on the 15 th day.In summary,the nano-silver FNP-Ag NC prepared in this study showed excellent antibacterial activity in the antibacterial experiment,and the use of blue LED light to obtain B-FNP-Ag NC nano-silver containing a large amount of active oxygen,further reducing the nano-silver The bactericidal concentration improves the antibacterial effect of nano-silver.The two nano-silver prepared in this study have very low toxicity to cells,and the nano-silver ointment prepared with the minimum bactericidal concentration achieves the purpose of promoting wound healing. |
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