Study On Chemiluminescence Detection Of Tumor Markers Based On Aptamer Technology

Global climate change and the aggravation of population aging are the root causes of people suffering from various diseases.Therefore,it is particularly important to develop accurate and sensitive detection platforms to diagnose markers of human-related diseases.In this paper,based on aptamer technology combined with catalytic chemiluminescent materials,a sensitive biosensor for detecting tumor markers has been developed by chemiluminescence,so as to provide cancer markers carcinoembryonic antigen(CEA),alpha fetoprotein(AFP)and adenosine triphosphate(ATP)for new detection methods and detection ideas.The specific research contents are as follows:(1)Dual aptamers combined with nickel nanoparticles to amplify the chemiluminescence signal at multiple sites to detect carcinoembryonic antigenSince CEA has the advantage of dual aptamers,we first used one of the aptamer chains through chelation,and in the case of histidine acting as a linking medium,the nickel nanoparticles and aptamers were combined together.Another aptamer chain modification is attached to the magnetic beads.In the presence of CEA,both aptamer chains will recognize the target simultaneously,and after magnetic separation,a distinct chemical signal will be detected due to the luminol chemiluminescence catalyzed by the nickel nanoparticles.In the absence of CEA,the chemical signal detected by it will be relatively low.CEA showed a good linear relationship with the chemiluminescence signal.The linear regression equation of CL intensity(ΔCL)and the logarithmic concentration of CEA(log c CEA)wasΔCL=968147.27+228249.72log c CEA,R~2=0.993.It calculated a minimum detection limit of 0.092 pg/m L.(2)Detection of carcinoembryonic antigen based on gold nanoparticles combined with double aptamer chemiluminescence methodOn the basis of the research in the first part,since streptavidin can be specifically recognized by its aptamer.Therefore,streptavidin-modified gold nanoparticles were constructed into dual-aptamer biosensors,and through the specific recognition of CEA by dual-aptamers,the dual-aptamer detection technology was successfully expanded in the field of chemiluminescence Applications.Gold nanoparticles have a good catalytic luminol chemiluminescence effect,thereby improving the sensitivity and specificity of detection.TheΔCL obtained in this biosensor has a good linear correlation with the logarithmic concentration of CEA(log c CEA).The linear regression equation isΔCL=12781.04+4032.37 log c CEA,R~2=0.997.It calculated a minimum detection limit of 0.122 pg/m L.This method expands the further development and application of dual aptamer technology and nanomaterials in the detection field.(3)Rapid detection of alpha-fetoprotein and adenosine triphosphate based on multifunctional DNA nucleic acid strand chemiluminescenceThe multifunctional DNA nucleic acid chain is based on the AFP and ATP adapter body and combined with its complementary double-stranded structure consisting of a single strand of DNA by complementary strand in AFP and adaptation of the ATP body modification biotin and G bases,using the AFP aptamer complementary strand modify mould avidin biotin combined with chain of horseradish peroxidase catalyzed luminol chemiluminescence-hydrogen peroxide system,ATP adaptor complementary chain modified G base and benformyl formaldehyde chemiluminescence,combined with magnetic separation technology,provides a good method and detection environment for AFP and ATP detection.The chemiluminescence values of AFP and ATP showed a good linear relationship,and the lowest detection line was 1.8 pg/m L and 0.5 n M,respectively.Compared with other detection methods,AFP and ATP showed a relatively wide detection range and low detection limit.