Extraction Of Silk Proteins And The Study Of Its Dispersion Behavior In Different Polymer Systems

Clinically,due to congenital malformations,trauma and other injuries,the patient’s partial activity function is lacking,which in turn seriously affects the patient’s normal life.Therefore,the repair and reconstruction of skin,muscle,bone tissue and other types of tissues has become one of the important problems in tissue engineering.For the defects of various tissue sites,it is of great significance to construct biological materials that meet the needs of biological functions.Silk protein is a special protein extracted from natural silk,which has excellent mechanical properties at the same time,but also has good biocompatibility,non-toxic and non-irritating,and it is easy to prepare,which has great potential in the field of biomaterials.In this paper,silk fibroin was selected as the research object to study its dispersion behavior in different polymer systems.First,the silk protein is extracted from natural silk;Secondly,the silk fibroin solution(SF)was combined with sodium alginate(SA),and a new type of silk protein/calcium alginate scaffold with a "reinforced concrete" structure was prepared using calcium ions as crosslinkers,and its physicochemical properties and biological properties were studied;then,the silk fibrous is combined with chitosan to prepare a silk protein/chitosan film with a mesh pattern and its physicochemical properties and biocompatibility are studied;finally,the filament protein fiber and polylactic acid are combined to prepare a Silk proteins/polylactic acid fibers with a "nuclear shell" structure and study their physicochemical properties and biocompatibility.Experimental results show that:(1)The mechanical properties of the traditional silk fibroin/calcium alginate scaffold are poor,and the fibroin/calcium alginate scaffold with a "reinforced concrete" structure prepared in this paper has more excellent mechanical properties than the traditional scaffold,and its compressive strength is about 7 times that of the traditional scaffold,and the compressive strength is 11.10±2.61 KPa and 72.68 ±6.73 Kpa,respectively.(2)The introduction of silk protein with mesh pattern in the pure chitosan film improved the mechanical properties of the material,the tensile strength increased by about 60%,the tensile strength was 7.58±1.53 MPa and 4.76±1.69 MPa,respectively,the elongation at break was increased by about 70%,the elongation at break was increased by28.22±3.57% and 16.6±2.30%,respectively.In vitro suture experiments showed that the introduction of silk fibrin fiber can improve the suture resistance of the film,and the silk protein/chitosan film can carry the weight of 10 g weight under the action of sharp suture lines.(3)The introduction of silk protein fiber in polylactic acid fiber improves the anti-fracture performance of fiber,and silk protein/polylactic acid fiber can still carry the weight of 2g weight in the case of polylactic acid fracture.