Application Of MOFs-based Stimuli-responsive Strategies In Biosensing And Photothermal Therap

Metal-organic frameworks（MOFs）is a type of novel crystal nanomaterials consists of metal ions and organic ligands which also called porous coordination polymers.MOFs-derived carbon materials is a kind of porous carbon materials using MOFs as templates.There are several merits using MOFs and MOFs-derived carbon materials as the materials for stimuli-responsive releasing strategy compared with other traditional porous materials such as larger pore size compared with microporous zeolite molecules;higher biocompatibility compared with mesoporous silica;simpler synthesis method and lower cost compared with traditional carbon materials.Stimuli-responsive releasing strategy was used in the fields of biosensing,drug-delivery and energy/gas storage.Many new ideas and methods are derived from this strategy.In this paper,a biosensor platform and a method of photothermal therapy were constructed based on stimuli-responsive releasing strategy,MOFs,and MOFs-derived carbon materials.The main research contents are as follows:1.An electrochemical biosensor based on MOFs,stimuli-responsive releasing strategy and signal amplification strategyMIL-101（Cr）is considered as an ideal material utilizing in amplifying signals.Its pore sized is about 2.3-2.7 nm and has 3D structure.We changed the organic ligand to synthesize MIL-101-NH₂（Cr）for encapsulating electroactive materials which enhanced the biocompatibility and preferable to biosensor.Furthermore,hydrothermal method is a simple and low-cost method.Using double-strand DNA as gatekeeper to encapsulate methylene blue（MB）into MIL-101-NH₂（Cr）,MB would release once the Nt.Bst NBI and p53 gene exists.The remining parts of DNA could form G-Quadruplex/Hemin DNAzyme to catalyze H₂O₂ and enhance the redox reaction of MB to prove electrochemical signals and obtain the purpose of detection p53 gene.2.Designing a photothermal therapy method based on ZIF-8 derived carbon materials and stimuli-responsive releasing strategyCarbroyxlated ZIF-8 derived carbon materials were broadly used in the fields of biosensing and cancer therapy and so forth.Utilizing carboyxlated ZIF-8 derived porous carbon materials（NCZIF）to encapsulate photothermal agents（MB or ICG）for phoththermal therapy is the thinking of this point.808 nm laser light could“open”the single-stranded DNA（ss DNA）linked on the materials viaπ-πinteractions and release photothermal agents.Synergistic effect was discovered owing to the photothermal effects exists not only on the photothermal agents but only on NCZIF.Single NCZIF could also release heat under 808 nm laser irradiation.Owing to superposition of heat,Cancer cells were killed successfully.