Preparation Of Polytetrahydropyrimidine Antibacterial Polymer And Study On Its Biological Activit

Bacterial infections in wounds often lead to the appearance of inflammation,and proper and effective treatment of bacterial infections is imperative.As the most commonly used antibacterial agents in clinical use,antibiotics have played a considerable role in the field of antibacterial since their advent,but as small molecule agents,they still face many challenges.For example,with the frequent and extensive use of antibiotics,some bacteria may gradually become less sensitive to certain antibiotics,leading to the abuse of antibiotics and the emergence of drug resistance.With the rapid development of technology,the requirements for antibacterial agents in the antibacterial field are gradually increasing,especially their antibacterial ability,anti-drug resistance ability and biocompatibility.In this experiment,we used alkyne monomers,monoamine monomers,formaldehyde and acetic acid as raw materials and synthesized a series of novel antibacterial polymers simply and efficiently through multi-component polymerization.Then we explored the physical and biological properties of these polymers and combined the polymers with hydrogels to expand their applications in the antibacterial field.The main research and conclusions are as follows:(1)12 polytetrahydropyrimidine polymers(P1-P12)were synthesized by changing the types of alkynyl monomers and monoamine monomers.The successful synthesis of polytetrahydropyrimidine polymers were proved by nuclear magnetic resonance spectroscopy,and the weight-average molecular weight of the polymers was between 4000-40000 by gel permeation chromatography;(2)The antibacterial zones of polytetrahydropyrimidine polymers on Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)were tested,the polymers with certain antibacterial ability were initially screened,and the polymers with ideal antibacterial effect were screened through the minimum inhibitory concentration(MIC)test.The MICs of polymers P3 and P4 against E.coli and S.aureus were 100 μg/m L,and the MIC of P6 against E.coli was 150 μg/m L;(3)Polytetrahydropyrimidine polymers with high biocompatibility were screened out by hemolysis and cytotoxicity tests.Polymer P4 did not show obvious hemolysis at 1000μg/m L,had excellent affinity on L929 cells and NIH3T3 cells at 100 μg/m L,and had no adverse effect on the proliferation of L929 cells;(4)The continuous antibacterial ability test proves that the same P4 solution can inhibit bacterial growth within 11 times of repeated contact with bacteria,the anti-drug resistance test proves that P4 can prevent bacteria from developing drug resistance within12 passages,and the continuous antibacterial ability and anti-drug resistance ability of P4 were better than that of double antibody(penicillin-streptomycin);(5)By changing the molecular weight of polytetrahydropyrimidine polymers,it is found that the antibacterial activity of polymer P4 enhanced with the increase of molecular weight,and the change of molecular weight will not affect its biocompatibility;(6)The application of polytetrahydropyrimidine polymers in the infection of open wounds in mice was tested,and the polymer P4 showed an excellent effect of promoting healing and anti-inflammatory in the infected wound model in mice;(7)Hyaluronic acid/gelatin hydrogel was prepared as the carrier of polytetrahydropyrimidine polymer and used in the treatment of open wounds,and the idea effect of promoting healing was achieved.