The Growth And Metabolism Of Bifidobacterium Animalis Subsp.lactis Probio-m8 Promoted By Lactobacillus Helveticus H9 In Fermented Milk

Probiotic fermented milk is a good carrier for delivering probiotics to human gastrointestinal tract.Compound probiotic fermentation can give fermented milk more probiotic functions.Based on the non-targeted metabonomics technology,the growth interaction and metabolism of probiotic Lactobacillus helveticus H9 and animal Bifidobacterium subspecies Probio-M8 in fermented milk were studied.The main results are as follows:1.Lactobacillus helveticus H9(1×10~6CFU/m L)and Bifidobacterium animalis subsp.lactis Probio-M8(1×10~6CFU/m L)were fermented in the ratio of 1∶1.At the end of fermentation(p H4.5),the viable number of Bifidobacterium animalis subsp.lactis Probio-M8 could reach(1.28±0.07)×10~8CFU/m L.Compared with single-strain fermentation,the number of viable bacteria of Bifidobacterium animalis subsp.lactis Probio-M8 increased by 2.33 times during compound fermentation.However,there was no significant difference in the growth of Lactobacillus helveticus H9.The results showed that the compound fermentation with Lactobacillus helveticus H9 could significantly promote the growth of Bifidobacterium animalis subsp.lactis Probio-M8.In addition,the fermentation time of compound fermented milk is 8 hours,which is significantly shorter and the acid production rate is faster than that of single-strain fermentation.2.Metabolic analysis of samples at 0 h,9 h and 12 h fermentation stages in a co-fermentation system of Lactobacillus helveticus H9 and Bifidobacterium animalis subsp.lactis Probio-M8.The results showed that the content and types of metabolites in compound fermentation were significantly higher than those in single bacteria fermentation,among which 5-methyl-2-hexanone,(E)-3-octen-2-one,acetic acid,alanine,3 The content of metabolites such as hydroxy-butyraldehyde in compound fermented milk was higher than that in single bacteria fermentation group.By analyzing the production pathways of metabolites,it is preliminarily deduced that Lactobacillus helveticus H9 may hydrolyze casein in fermented milk to produce small molecular peptides,alanine,tyrosine and other substances.This way maybe can promote Bacteria subsp.lactis Probio-M8 grown.This article provides a theoretical basis for the development and utilization of compound probiotic fermented milk by studying the growth and metabolism of Lactobacillus helveticus H9 and Bifidobacterium animalis subsp.lactis Probio-M8 in fermented milk.