Electrochemical Analysis Of Biothiol Based On Bluetooth Wireless Sensing Method And Its Application

L-homocysteine(L-Hcy)and L-cysteine(L-Cys),which are present in normal mammalian intra-and extracellular fluids,are involved in signaling,protein modification,cell differentiation and other physiological activities,have an integral role in numerous physiological processes in the body,and are closely associated with the development and progression of numerous diseases.Therefore,the development of new methods for the detection of L-Hcy and L-Cys in organisms is of great importance for the prevention and treatment of the above diseases.Based on the extended gate field effect transistor(EGFET)and embedded Bluetooth technology,a new signal amplification electrochemical wireless Bluetooth transmission system is constructed,combined with the self-assembled 3,3’-dithiobis(1-propanesulfonic acid)(SPS)-Hg2+ composite membrane modified gold electrode(Au/SPS/Hg),to achieve high sensitivity detection of L-Cys and L-Hcy.The main research contents are as follows:1.Construction of wireless bluetooth sensing system based on chip gold electrode.On the basis of bluetooth low energy,the hardware of the potentiometric embedded monitoring system is selected and combined,and the software is also developed,and a new Bluetooth wireless sensing detection system is constructed in combination with the extended gate field effect transistor.The simulation test of the wireless sensing system and the analysis of the actual electrochemical detection show that the data transmission of the sensing system is stable,and the potential value fluctuates between ±0.0005 V,which meets the precision requirements of the required 0.001 V;and the signal can be amplified in situ in real time by three times,which improves the signal-to-noise ratio and improves the detection sensitivity of the electrode to a certain extent,and the sensing system will further expand the application range of electrochemical detection methods.2.L-cysteine electrochemical monitoring and application based on bluetooth wireless sensing.Firstly,the self-assembly method was used to assemble and modify the 3,3’-dithiobis(1-propanesulfonic acid)membrane on the chip gold electrode,and then combined with mercury ions to form an Au/SPS/Hg modified electrode.Through the combination of the extended gate field effect transistor and the bluetooth wireless transmission module,a new type of signal amplification electrochemical bluetooth wireless sensing system is constructed.The bluetooth wireless sensing system based on Au/SPS/Hg detects L-Cys in a linear range of 5.0×10-6-1.0×10-3 mol/L,the linear equation △E=-193.561gc-1089.08,and the lower detection limit is 2.53 μmol/L.The wireless sensing system was successfully applied to the online detection of L-Cys in pig serum,pig chyme and its peritoneal fluid,realizing the dynamic data monitoring and in vivo testing of L-Cys in the pig breeding process,and has important application value in the fields of livestock health breeding and biomedical diagnosis.3.L-homocysteine electrochemical monitoring and application based on bluetooth wireless sensing.The bluetooth wireless sensing system based on Au/SPS/Hg composite membrane modified electrode was carefully explored in the electrochemical response performance of L-Hcy,and the electrochemical test performance parameters were optimized,and the linear range was determined to be 5.0×10-7～1.0×10-4 mol/L,the linear equation △E=-136.81gc-908.80,and the lower detection limit was 0.21 μmol/L.The electrode detection system is responsive,reproducible and stable.The effective distinction between L-homocysteine and L-cysteine is realized by potential testing,and the separate detection of two thiol substances can be realized,and it is expected to realize the selective simultaneous detection of L-Hcy and L-Cys,which has important application value in the fields of livestock health breeding and biomedical diagnosis.