Construction And Osteogenic Properties Of Biofunctionalized Elastic Oriented Cellulose Scaffolds

The traditional scaffolds of bone tissue engineering are generally deficient in source,oriented structure,and antibacterial,osteogenic and angiogenic activity.Natural wood has the multi-scale layered anisotropic and directional porous structure very similar to bone,which has the potential to be developed as bone repair scaffold material.To this end,a class of highly elastic oriented cellulose scaffolds(EW)with the highly anisotropic and oriented porous structure were constructed by chemical treatment and freeze-drying of natural balsa wood,and the effects of EW scaffolds with different mechanical strength and modulus on cell behaviors such as proliferation,adhesion and spreading were explored.The results showed that EW scaffolds had good cytocompatibility,and the cells showed better cell morphology and proliferation ability on EW scaffolds with moderate mechanical strength and modulus.Based on this,polydopamine(PDA)was further utilized to load chitosan quaternary ammonium salt(CQS)and dimethyloxaloylglycine(DMOG)onto EW scaffolds to obtain biofunctionalized EW scaffolds(EW@P-C-D)with both antibacterial,osteogenic and angiogenic abilities.The experimental results showed that the EW@P-C-D scaffold not only has excellent bactericidal and bacteriostatic ability,but also has good cytocompatibility and non-toxicity.It can also significantly facilitate the secretion of alkaline phosphatase(ALP)and the formation of calcium nodules,effectively promote the formation of tubule network,and significantly upregulate the expression level of related osteogenic and angiogenic genes.This kind of EW@P-C-D scaffolds,which has the characteristics of natural renewability,highly anisotropic structure,and antibacterial,osteogenic and angiogenic activity,can be expected to be an ideal novel scaffold material for bone repair.