Study On Process,Structural Characterization And Stability Analysis Of Loading Of Vitamin E By Starch-based Carrier

Vitamin E（V_E）,as a fat-soluble nutrient which is essential for human health.It is needed to be loaded and protected,because its chemical property is unstable and it is susceptible to light,oxygen,ultraviolet and other effects during processing and storage.Starch is widely used in food,medicine and chemical industry because of its wide source,low price and biodegradability.In this paper,loading of V_E by three different starch-based carrier was explored,which laid a theoretical foundation for the application of starch-based carrier to load fat-soluble nutrients.The main research contents and results are as follows:Amylose can use helical cavity to include fat-soluble guest molecules.Debranched starch was prepared from high amylose corn starch by enzymatic hydrolysis,and then V_E was incorporated.With the content of V_E as the response value,the optimal inclusion parameters were determined by single factor and orthogonal experiments,then the structure of the inclusion complex was characterized.The optimal conditions for preparing debranched starch/V_E inclusion complex were25℃for reaction temperature,30%for V_E addition,4 h for reaction time,and the content of V_E was 178.34 mg/g.The results of XRD showed that the complex generated a weak diffraction angle of B+V.It is found that the storage and moisture stability of inclusion complex were stronger,and the protective effect on V_E was better.Conventional starch microspheres are mainly prepared by method of chemical crosslinking,and load drug by adsorption.In this study,W/O emulsion was prepared using milling waxy corn starch as raw materials,V_E as oil phase.Then starch aggregate loaded with V_E was prepared by the method of ethanol deposition.With the content of V_E as the response value,the optimal preparation parameters of starch aggregate were determined by single factor and response surface experiments.The results showed that the optimal conditions were 8.6 h for milling time,90%for water addition,5 h for reaction time,and the content of V_E was 105.48 mg/g.The morphology of starch aggregate was irregular spherical,and the crystal structure was A type.The initial phase transition temperature was lower than that of starch-V_Eadsorbate,but it could significantly improve the storage stability of V_E.Octenyl succinic anhydride modified starch is an esterified starch.It loads V_E by emulsification embedding.However,V_E is unstable due to its poor film-forming.Modified octenyl succinic anhydride modified waxy corn starch（OSS）film was prepared via trisodium trimetaphosphate modification of enzyme-hydrolyzed OSS.The modification process was optimized by single-factor experiment and response surface methodology with tensile strength of film as response value,and the structure of modified OSS films obtained under the optimal conditions was characterized by³¹PNMR and FTIR.The results showed that under the optimal conditions of reaction time 5.8 h,p H 6.8,trisodium trimetaphosphate 18%of OSS mass,the tensile strength of modified OSS film was 2.19 MPa,69.77%higher than that of the OSS film without modification.Meanwhile,modified OSS film displayed a more homogeneous cross section with onset phase transition temperature increased by 14.6℃,which could be attributed to the hydrogen bond formed between trisodium trimetaphosphate and OSS.Moreover,it was found that trisodium trimetaphosphate modification of OSS loaded with V_E could effectively improve the storage stability of V_E.