Construction And Properties Study Of Polysaccharide-Surfactant Carrier

The use of surfactants to construct drug carriers has been widely studied.The surfactants can self-assemble at certain concentration and formed microemulsion,liquid crystal and so on,and polysaccharide has attracted attention due to its good biocompatibility.In this thesis,nonionic surfactant Polyoxyethylene dehydrated sorbitan monooleate（Tween 80）,Tween 80 and gellan gum were selected to construct aggregations,and polyphenol drugs were loaded by drug carrier,and the molecular interaction was studied by infrared spectroscopy.The free radical scavenging method was used to study the antioxidant activity and the drug release behavior was discussed.This thesis included the following parts:1.In this chapter,mainly reviewed the drug loading performance of hydrogels constructed by polysaccharides as drug carriers,the performance of self-assembled aggregations of surfactants as drug carriers,and the research progress of aggregations constructed by polysaccharides and surfactants as drug carriers,including polysaccharide-micellar aggregations,polysaccharide-microemulsion aggregations and the interaction between polysaccharides and surfactants.Therefore,on the basis of previous studies,Tween 80 and Gellan gum were mainly selected to construct aggregations to study the antioxidant properties and release properties of drug-loaded aggregations.2.In this chapter,Tween 80/polyethylene glycol 400（PEG 400）/H₂O was used to construct aggregations.On this basis,1,2 propylene glycol and isopropyl myristate（IPM）were introduced to study the phase behavior of the system,curcumin was loaded in each carrier.For the samples of S series,the IC₅₀ of curcumin in the carrier was one order of magnitude lower than that in ethanol carrier,and showed good antioxidant properties.The release curve of curcumin in carrier conformed to the first order kinetic equation,which belongs to the concentration-controlled diffusion release.With increase in water content,during the study release time,the maximum cumulative release rate decreased.Then agarose was introduced to construct aggregation to study the drug release behavior of curcumin.In the Tween 80/PEG 400/H₂O/agarose system,we found that p H affected the release rate of curcumin.In the Tween 80/1,2 propylene glycol/PEG400/IPM/H₂O/agarose system,The release rate and maximum cumulative release rate of curcumin decreased with the increase of agarose content and the decrease of S+O content.3.In this chapter,Gellan gum and Tween 80 were mainly used to construct aggregations.By adding different contents of GG into the system,combining with lecithin,changing the oil phase（ethyl oleate,sunflower oil,soybean oil）,changing the mass ratio of components,changing the temperature of the external environment and the p H of the releasing medium,the release behavior of curcumin in the carrier was studied.After the introduction of GG,It took longer for curcumin to reach its maximum cumulative release rate.Then the release behavior of apigenin and dihydromyricetin in the carrier was studied.The interaction between Tween 80/GG system and Tween 80/lecithin（SL）/GG system was studied by surface tension method.The results showed that the GG content,the molar ratio of Tween 80 and SL,and the external temperature affect the critical micelle concentration and other surface activity parameters of the mixed system.Withthe increase in temperature,the critical micelle concentration of the system decreased.4.In this chapter,Tween 80 and Polyoxyethylene laurel ether（Brij 35）as surfactant phase,and Gellan gum（GG）was introduced to construct aggregations for containing curcumin.The effects of Tween 80 and Brij 35 mass ratio,surfactant phase/oil ratio,GG content and temperature on curcumin release behavior were studied.Surface tension method was used to study the interaction between Tween 80/Brij 35 mixed system and Tween 80/Brij 35/GG mixed system.