The Effect Of Frequent Consumption Of Takeaway Food On The Gut Microbiota Of Adults

Objective:In this study,a cross-sectional study was used to detect the difference of the gut microbiota community of frequent takeaway food consumers by using 16 S ribosome DNA(16S ribosome DNA,16 S r DNA)high-throughput sequencing technology,and to compare the difference of gut microbial communities with those of long-term home eaters.To elucidate the effect of takeaway food on the gut microbiota of adults.Methods:From February 2021 to February 2022,recruit 30 research subjects who meet the standards of the takeaway group,and recruit 30 research subjects who meet the standards of the non-takeaway group.The age,gender,height,weight,BMI,past history,diet and defecation habits,and fecal samples were collected from the research subjects,and 16 S r DNA high-throughput sequencing technology was used to quantitatively and qualitatively analyze the gut microbiota in the collected 60 fecal samples.The differences and diversity of gut microbiota at various biological taxonomic levels,and functional annotation through KEGG to explore the possible health effects of takeaway food.Results:(1)Compared with the non-takeaway group,the number of OTU of gut microbiota in the takeaway group was higher.(2)The Alpha diversity of gut microbiota in the takeaway group and the non-takeaway group were similar,But the significant differences in Beta diversity suggest that there are structural differences in the gut microbiota between the two groups.(3)Compared with the non-takeaway group,the F/B ratio of the takeaway group decreased,At the family level,the abundance of Prevotellaceae in the takeaway group was significantly higher than that in the non-takeaway group,and the abundance of Bacteroidellaceae and Bifidobacterium was lower than that in the non-takeaway group;At the genus level,the abundance of Prevotella in the takeaway group was significantly higher than that in the non-takeaway group,and the abundances of Bacteroides and Faecalibacterium were lower than those in the non-takeaway group。At the species level,the abundances of Prevotella copri and Prevotella stercorea in the takeaway group were significantly higher than those in the non-takeaway group,and the abundances of Faecalibacterium prausnitzii,Bacteroides uniformis and Bacteroides ovatus were significantly lower than those in the non-takeaway group.(4)The functional annotation of the two groups of gut microbiota genes through the KEGG pathway and the differences were compared,and there were differences in the abundance of human disease genes at the level1;at the level2,nucleotide metabolism,amino acid metabolism,and lipid metabolism genes There are differences in abundance;There are differences in the pathways of D-glutamine and D-glutamate metabolism,pyrimidine metabolism,riboflavin metabolism,purine metabolism,NODlike receptor signaling pathway,biotin metabolism,secondary bile acid metabolism,glycosaminoglycan degradation,and primary bile acid biosynthesis.Conclusion:Dietary differences caused significant differences in the gut microbiota structure between the two groups.Frequent consumption of takeaway food leads to an increase in the number of gut microbiota species in adults.But takeaway food led to a significant increase in the abundance of Prevotella in the gut microbiota of adults,while the abundance of beneficial bacteria significantly decreased.the gut microbiota disturbances induced by takeaway food and the metabolic pathways with significant differences were closely related to the occurrence of NAFLD,IBD,CRC and other diseases.