Preparation,Antibacterial Properties And Mechanisms Of Novel Copper Telluride Nanomaterial

Antibiotics are one of the most important methods to treat bacterial infections,but the problem of bacterial drug resistance caused by the overuse of antibiotics is becoming increasingly serious.Therefore,there is an urgent need to develop new antimicrobial agents and seek new therapeutic strategies to address bacterial resistance.Antibacterial nanomaterials have been more appealing due to their unique antibacterial mechanism and rare resistance.However,bactericidal nanomedicines often suffer from a complicated design and synthesis process or insufficient intrinsic sterilization efficiency.In view of the above problems,a novel copper telluride antibacterial nanomaterial was prepared,which has the advantages of simple preparation,strong intrinsic bactericidal activity,and unique antibacterial mechanism.I.Preparation and characterization of novel copper telluride nanomaterial.Cu Te nanomaterial was prepared by a "one-pot" strategy at room temperature and observed by TEM and SEM as "grape cluster-like" nano-clusters with a rough surface.In addition,the basic properties of Cu Te such as crystal form,valence state,particle size and Zeta potential were obtained by XRD,XPS,ICP-OES,and particle size and Zeta potential measurements.II.Evaluation of antibacterial properties of novel copper telluride nanomaterial in vitro and in vivo.In vitro antibacterial experiments showed that Cu Te exhibited strong antibacterial activity against E.coli and Acinetobacter baumannii even at lower concentrations,and significantly inhibited the growth of S.aureus at increased concentrations.Meanwhile,Cu Te can effectively break the established biofilm barrier and inhibit the formation of bacterial biofilm significantly.The results of in vivo antibacterial experiments showed that Cu Te significantly reduced the number of E.coli colonizing in the abdominal cavity and organs of mice with abdominal infection.In addition,Cu Te increased survival rate to 90% in mice survival assay,which demonstrated the effectiveness of Cu Te in treating bacterial infections in vivo.III.Antibacterial mechanism and biocompatibility of novel copper telluride nanomaterial.Cu Te maximizes the bactericidal efficiency of ROS through the synergistic mechanism of its own binding to bacteria,production of reactive oxygen species(ROS)and consumption of glutathione(GSH),which may be the main reason for the strong intrinsic bactericidal activity of Cu Te.At the same time,after treating bacteria with Cu Te,various intracellular components including nucleic acids,proteins,and potassium ions leaked.In addition,hemolysis and animal toxicity experiments also provided preliminary evidence for the biocompatibility of Cu Te.In summary,the novel copper telluride nanomaterial prepared in this study has the advantages of simple preparation process,mild preparation condition,strong antibacterial activity,and unique bactericidal mechanism,which provides more options for the development and use of new antibacterial agents.More importantly,this finding could provide new ideas for the development of inorganic nano-agent with simplified structure,multiple antibacterial mechanisms,and efficient bactericidal properties.