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Molecularly Imprinted Solid Phase Microextraction Fiber Coating Extraction And Analysis Of Multiple Environmental Estrogens In Wate

Posted on:2024-09-24Degree:MasterType:Thesis
Country:ChinaCandidate:Y L DuanFull Text:PDF
GTID:2531307112954399Subject:Analytical Chemistry
Abstract/Summary:PDF Full Text Request
As a class of chemicals that can interfere with endogenous hormones,environmental estrogens pose a threat to human health.They are widely used and can be continuously released into environmental water through a variety of pathways.There are problems such as complex sample matrices,numerous interfering substances,and low levels of target compounds when monitoring a variety of environmental estrogens in environmental waters.It is essential to pre-treat the samples to dissolve the above problems.Based on the above,a solid phase microextraction fiber with multiple specific recognition sites was developed and the molecularly imprinted fiber array was constructed for the selective enrichment analysis of various environmental estrogens in environmental water.There are two aspects of the main research described below.(1)A multi-site molecularly imprinted solid phase microextraction fiber was developed.Coupling solid phase microextraction technique with a multi-template imprinted strategy triggered off durable and sensitive molecularly imprinted solid phase microextraction fibers with multi-site recognition.Various characterization tools have been used to characterize the synthesized multi-site molecularly imprinted fibers.Then,the sorption properties and service life were also investigated under the optimum extraction conditions.In the concentration range of 100-4000 μg/L,the amount of three analytes extracted using this imprinted material was 182-1479 folds higher than that using commercial polyacrylate fibers.And there was no significant decrease in the extraction amount after 100 cycles of extraction.For the detection of multiple crossclass environmental estrogens in the water samples,a multi-site molecularly imprinted solid phase microextraction analytical method was developed.The method showed lower detection limits(0.003-0.02 μg/L),wider linearity(0.01-200 μg/L)and higher enrichment factors(118.56-224.33),allowing sensitive monitoring of multiple different classes of environmental estrogens in complex environmental water samples simultaneously.(2)A molecularly imprinted fiber array was constructed.There were five different imprinted fibers synthesized using methyl paraben and its four main metabolites as template molecules,respectively,and multiple imprinted fibers were assembled to form different molecularly imprinted fiber array extraction devices.Compared to single imprinted fiber,the molecularly imprinted fiber array offered superior extraction performance.In addition,the molecularly imprinted fiber array showed better enrichment performance.The peak areas of five compounds at low concentrations was4-1072 folds than that by the three commercial arrays.The service life can reach at least100 cycles.Further molecularly imprinted fiber array was applied to targeted monitoring for identifying methyl paraben and its four major metabolites in environmental water samples.The method achieved a limit of detection of 0.033 μg/L for methyl paraben and its major metabolites in environmental water samples with the recoveries ranging from 86.78 to 113.96% and the relative standard deviations between0.75 and 9.17%.In particular,the detection amount of benzoic acid was 0.43 μg/L.Consequently,the proposed molecularly imprinted fiber array provides an effective approach for sensitive monitoring of multiple environmental estrogen residues in environmental water samples,significantly reducing matrix interference and enhancing the enrichment effect.
Keywords/Search Tags:Molecular imprinting technology, solid phase microextraction, environmental estrogens, selective adsorption, environmental water samples
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