Study On Surface Modification And Performance Of Carrier For Protein Adsorption

The protein adsorption behavior on the surface of the medical carrier is crucial for its application in the medical field.However,the wettability and biocompatibility of the surface of many carrier materials are difficult to meet the requirements of the carrier surface in the field of protein adsorption,which may easily lead to adverse consequences such as conformational changes of the protein adsorbed on the surface.Due to their own properties,polyelectrolytes can effectively solve the above problems.And it was widely used in the surface modification of materials.Therefore,this paper carried out study on carrier surface modification for protein adsorption,that is,designing a modification process suitable for polyelectrolyte layers on the surface of various carrier materials,studying the properties of the modified surface and protein adsorption behavior,and revealing the surface modification mechanism.The results provide a good technical reference for the surface modification of carrier materials in the field of protein adsorption.Firstly,polydimethylsiloxane(PDMS)was used as the substrate to study the polymerization process of dopamine(DA)in acidic conditions.That is,the influence of process parameters on the surface properties of DA after polymerization was explored by wettability test and protein adsorption test,and the biocompatibility of the surface was also studied by cell activity test.The results show that the amount of DA polymerization is positively correlated with the environmental p H,temperature and initiator concentration.The rational selection of polymerization process parameters can meet the requirements of low-cost and high-performance surfaces.PDA has excellent wettability,water stability and biocompatibility.The cell survival rate of mouse osteoblast MC3T3-E1 cultured in PDMS extract after deposition was 80.27%,much higher than 67.38%in the original PDMS extract,which is an ideal secondary reaction platform.This also lays a foundation for the development of one-step polymerization co-deposition strategies in acidic conditions.Secondly,based on the polymerization of DA in acidic conditions,polyelectrolyte coating was obtained by co-depositing DA and acrylic acid(AA)monomers using one-step polymerization co-deposition strategy.The wettability,protein adsorption and responsiveness of the coating were analyzed to characterize the modified surface properties,revealing the protein adsorption mechanism on the surface.The results showed that the PDA/PAA polyelectrolyte layer could greatly reduce the water contact angle of the modified surface,and the protein adsorption amount of the modified surface increased by 57.74%compared with the unmodified surface.The wettability and protein adsorption of the polyelectrolyte layer showed a high correlation with the p H of the adsorption environment.That is,when the adsorption environment p H was 8,the protein adsorption amount of the coating was only 15.55%of the protein adsorption amount when the p H was 5.Finally,chitosan natural polyelectrolyte coating was obtained by one-step polymerization co-deposition strategy and self-assembly process.The effect of chitosan concentration on the surface properties of the coating was studied through the wettability and protein adsorption properties of the coating.The results show that the concentration of chitosan has a significant effect on the roughness of the modified surface and the thickness of the coating.The surface water contact angle increases first and then decreases with the increase of chitosan concentration.The chitosan polyelectrolyte layer also has good protein adsorption,and the protein adsorption capacity on modified surface is 580.50 ng/cm~2,which is 4.43 times higher than that of the unmodified surface.In addition,the cell survival rate of MC3T3-E1 cells cultured for 72 h in the chitosan self-assembled PDMS extract increased to 102%,showing that chitosan has a positive effect on promoting the growth and proliferation of MC3T3-E1 cells.Therefore,the polyelectrolyte coating prepared in this paper can effectively improve the biocompatibility of the material surface and broaden its application range in biomedical field.The above research results will provide better technical guidance for the development of surface modification strategies for protein adsorption.