The Alleviating Effect Of Roxburghii Polysaccharide On Ulcerative Coliti

In recent years,ulcerative colitis(UC)has become a global disease with increasing incidence.At present,the drug measalazine for UC has side effects such as nausea and vomiting,gastrointestinal bleeding and rash.Studies have shown that dietary polysaccharides can alleviate UC-related symptoms through multiple pathways.As a characteristic fruit,Rosa roxburghii tratt is rich in a large number of polysaccharide active ingredients,and at this stage,whether Rosa roxburghii polysaccharide(RP)has a mitigating effect on UC has not been reported.Therefore,this study mainly discusses the remission of RP on UC mice and the regulation of intestinal microbiota,which provides a basic basis for the development of functional Rosa roxburghii tratt products,and the main results are as follows.(1)Regulatory effect of Rosa roxburghii polysaccharides on mice with ulcerative colitis.The UC model was established by dextran sodium sulfate(DSS)induction,and RP was used as raw material to explore the regulatory effect of RP on UC mice.The results showed that RP could increase body weight,colon length and DAI index,improve colon histopathological injury,improve antioxidant capacity in serum and colon tissue,reduce inflammatory response in serum and colon group,and improve the expression of tight junction proteins in the intestinal barrier compared with the model group.(2)Effect of Rosa roxburghii polysaccharides on intestinal flora of mice with ulcerative colitis.16S r RNA and non-targeted metabolomics were used to analyze the composition and diversity of intestinal microbiota and its metabolites,and explore the effect of RP on the intestinal microbiota of UC mice.The results showed that RP could reduce the relative abundance of Firmicutes,Proteobacteria and Actinobacteria at the gate level and increase the relative abundance of Bacteroidetes compared with the model group.At the genus level,RP increases the relative abundance of beneficial bacteria such as Akkermansia,Bifidobacterium,Lactobacillus and Turicibacter,and decreases the relative abundance of harmful bacteria such as Oscillibacter,Sutterella,Desulfovibrio and Ruminococcus.Increase the content of acetic acid,propionic acid and butyric acid in short-chain fatty acids(SCFAs).Therefore,it is speculated that RP may alleviate UC by regulating the intestinal microbiota and increasing SCFAs content.(3)Relief effect of fecal bacteria transplantation(FMT)Rosa roxburghii polysaccharide-regulated intestinal microbiota on mice with ulcerative colitis.The fecal microorganisms of RP donors were gavaged to cocolitis mice in FMT to explore the remission effect of fecal microorganisms from RP donors on UC mice.The results showed that compared with the model group,the fecal microorganisms of the transplanted RP mice could also alleviate weight loss,colon shortening,DAI index increase,colon tissue damage,oxidative stress damage,anti-inflammatory effect,and intestinal barrier damage in UC mice.In terms of intestinal microbiota,the relative abundance of harmful bacteria such as Oscillibacter,Shigella,Desulfovibrio and Ruminococcus was reduced by increasing the relative abundance of beneficial bacteria such as unidentified S24-7,Allobaculum,Odoribacter and Turicibacter.Increase the content of SCFAs such as acetic acid,propionic acid and butyric acid to regulate intestinal microbiota disorders in mice.In summary,RP can alleviate DSS-induced colitis by reducing oxidative stress damage,improving inflammatory response,reducing LPS level and increasing the expression of tight junction protein,reducing intestinal barrier damage,and regulating intestinal microbiota increasing SCFAs content.