| As one of Chinese main crops,pea is rich in protein,fiber,vitamins and macronutrients,regular intake of pea can reduce the risk of diabetes,cancer and cardiovascular diseases.Natural pea components have severely limited their application,development,and high-value product exploitation,based on this background,in this study,the pea components(PA and PDF)were modified by dynamic high pressure microfluidization(DHPM)technology;and then to form binary and ternary complexes with chlorogenic acid(CA)and pea components,was aimed to reveal the changes in the structural and functional properties of pea components and their complexes under different treatment pressures,as well as the modification mechanisms of pea component in different systems.The modification of PA and pea albumin-chlorogenic acid(PA-CA)physicochemical properties and functional characteristics by DHPM showed that the particle size and zeta potential of PA first decreased and then increased after treatment;the microstructure,secondary structure and tertiary structure of the modified albumin were both changed;the solubility of albumin with small particle sizes significantly improved by shear force and other effects,moreover,and the emulsification and foaming characteristics were also enhanced.In the system of PA-CA complexes processed by DHPM,the microenvironment of tryptophan in album was changed by CA,resulting in significant changes in the albumin’s structure,and a significant reduction in particle size and solubility compared to the albumin group.Compared to the untreated complexes,the particle sizes of the treatment group were significantly reduced,and the absolute value of the zeta potential was also changed;moreover,the solubility,emulsification and foaming properties of the complexes were improved to some extent.In this study,PDF was modified by DHPM technology with the aim to reveal the modulation of the structural and adsorption properties of PDF under different pressures.In addition,PDF-CA after DHPM treated were obtained,to further reveal the interaction between PDF and CA,and synergistic action of CA and DHPM on PDF.Due to the molecular interplay of PDF-CA,the results showed that PDF and PDF-CA samples with different particle sizes could be obtained after DHPM treatment.Microscopic evaluation of PDF and PDF-CA also showed significant morphological changes;the crystallinity of both PDF and complexes decreased during the treatment,but the trend of thermal stability showed a different shift.In addition,the DHPM treatment made the samples exhibit better water-and oil-holding properties,enhanced their ability to bind glucose and cholesterol,and gradually enhanced the adsorption capacity with increasing pressure.These results illustrated that the structural and adsorption properties of PDF could be effectively modified by DHPM and CA.The ternary complexes were prepared by PA,PDF and CA,and modified by DHPM technique.The effects of DHPM on the structural properties of PA-PDF-CA ternary complexes and the characteristics of Pickering emulsions stabilized by them were investigated.It was shown that the emulsions prepared from modified complexes could exhibit better interfacial properties,and the addition of CA could enhance the antioxidant activity of the whole system.On this basis,the PA-PDF-CA complexes were used as raw materials to obtain stable Pickering emulsions to investigate the effects of DHPM technology on the structural properties of the pea ternary complexes,and the properties of the Pickering emulsions stabilized by them.The results showed that the particle sizes of the complexes decreased with the increase of treatment pressures;the emulsions prepared by the modified complexes had good antioxidant capacity compared with the untreated samples,as evidenced by the significantly higher scavenging rates of DPPH and ABTS radicals;the results of the rheological behavior indicated that the emulsions showed shear thinning characteristics,and the DHPM imparted better apparent viscosity and temperature stability to the emulsions. |
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