Alleviating Effect Of Camel Milk On Non-alcoholic Fatty Liver Disease In Mice

Non-alcoholic Fatty Liver Disease(NAFLD)is a worldwide prevalent community health disease that is notably characterized by severe hepatic lipid accumulation and disturbances in the body’s glycolipid metabolism.As a natural source of high quality milk,camel milk has been shown to have hypoglycemic,anti-inflammatory and hepatoprotective effects and modulate intestinal flora.The aim of this study is to investigate the alleviating effect of camel milk on high-fat diet-induced NAFLD in mice by using its nutritional properties,and to investigate its alleviating mechanism by assessing the glycolipid metabolism,liver lipid profile and intestinal microbial diversity in mice,with the aim of providing an experimental basis for developing camel milk into a relevant functional product.The study results are as follows:In this study,the NAFLD model was established by feeding C57BL/6J mice a high-fat diet for 12 weeks,and insulin resistance,four indices of blood lipids,inflammatory response and hepatic lipid accumulation were detected to assess the glucolipid metabolic capacity of mice.The results showed that camel milk reduced insulin resistance,improved blood lipid status,reduced inflammatory response,decreased lipid accumulation in the liver,and protected the structural integrity of hepatocytes and liver lobules in mice.Various lipid biomarkers,including choline,cardiolipin,triglyceride,phosphatidylcholine and lysophosphatidylcholine,were screened by analyzing the liver lipidomic from each group of mice;camel milk reduced lipid accumulation in the liver of NAFLD mice by enhancing its involvement in sphingolipid metabolism,glycerol ester metabolism and glycerophospholipid metabolism.Intestinal microbial sequencing results demonstrated that camel milk significantly increased the diversity of microorganisms in the feces of NAFLD mice and effectively regulated the structure of intestinal flora.The relative abundance of beneficial bacteria genus Bacteroides,norank＿f＿Muribaculaceae and Alloprevotella,which were negatively correlated with body weight,blood glucose,serum and liver TG level in the feces of mice in the camel milk group,was significantly up-regulated.The relative abundance of the harmful bacteria genus Dubosiella and Coriobacteriaceae＿UCG-002,which were positively correlated with body weight,blood glucose,serum and liver TG level in the feces of mice in the camel milk group,was significantly down-regulated.Camel milk has an alleviating effect on hepatic lipid accumulation in NAFLD mice,and this alleviating effect is associated with enhanced glycolipid metabolism and improved intestinal microbial ecology in mice.