Preparation And Biological Application Of Tellurium-based Photothermal Conversion Materials

Tellurium nanomaterials have attracted great attention in the field of photothermal diagnosis and treatment due to their semiconductor properties.In this paper,a series of tellurium nanomaterials were obtained by adding different end-sealing agents under mild conditions,and a butterfly shaped tellurium nanoparticle was screened out,which was loaded with photosensitizer Ce6.A collaborative antibacterial platform was constructed,and the antibacterial effect was evaluated in vitro and in vivo.The following are the specific research contents:Firstly,tellurium nanomaterials with different morphologies were synthesized by mild reaction conditions using Na₂TeO₃ as precursor and by adjusting the types of sealing agents.The morphology,composition,crystal phase and absorbance range of the particles were characterized by TEM,XRD,XPS and UV-vis.The size distribution of nanoparticles was measured by dynamic light scattering（DLS）method.Secondly,the tellurium nanomaterials with the highest photothermal conversion efficiency were chosen after their photothermal characteristics were examined by an 808nm near-infrared laser.The results demonstrate that when fructose is employed as a capping agent and glutathione as a reducing agent in a water bath at 60°C for 4 h,fructose-tellurium（FTe）nanoparticles have the best photothermal conversion efficiency.DLS evaluated the stability of FTe in PBS and ultra-pure water.Subsequently,a combined photothermal（PTT）and photodynamic therapy（PDT）system（FTe-Ce6 NPs）was constructed using FTe as a carrier loaded with photosensitizer Ce6.Finally,the efficacy of antibacterial treatment of FTe-Ce6 NPs in vitro and in vivo was evaluated.Cytotoxicity test confirmed that FTe-Ce6 NPs had low biotoxicity.The results of plate counting in vitro and wound healing experiment in vivo showed that irradiation of FTe-Ce6 NPs with 808 nm near infrared laser could kill 99.9%of bacteria,indicating that the constructed collaborative treatment platform has good bacterial inhibition effect,and can effectively achieve sterilization and promote wound healing.