Correlation Between The Rat’s Vitro And In Vivo And The Active Ingrdient Were All Affected By The Leaching Conditions

In order to optimize the composition of Salvia of compound Salvia tablets by extraction conditions,the experimental change the the Salvia leaching conditions were investigated Tanshinone of the compound Salvia and salvianolic acid leaching,and Tanshinone compound Salvia and salvianolic acid a comparative study of the pharmacokinetics in rats under the curve,the AUC.Salvia active ingredient in vitro leaching and in vivo uptake data as a basis for the in vivo correlation of evaluation,to determine the optimum leaching conditions.Study are as follows:1:under different leaching conditions fufangsalvia tablet active ingredient content determination:a system of law were contained in the "Chinese Pharmacopoeia" Compound Salvia Tablets basis,respectively,changing the ethanol concentration,solvent amount,returning three extraction conditions,tanshinone and salvianolic acid composition by high performance liquid chromatography,and determination of the content of the target composition.The results showed that:Pharmacopoeia compound Tanshinone Ⅱ A,salvianolic acid B content is 1.0446 mg/g,31.78 mg/g,;change extraction conditions I:30%ethanol concentration,solvent volume doubled,the extraction time 1.5h,tanshinone Ⅱ A,salvianolic acid B content of 1.02 mg/g,24.17 mg/g.Conditions Ⅱ:70%ethanol concentration,solvent amount,extraction time 1.5h,tanshinone Ⅱ A,salvianolic acid B were 1.155 mg/g,23.04 mg/g;changing the process ⅡI:50%of the ethanol concentration,solvent volume 3 times,extraction time 1.5h,tanshinone ⅡA,salvianolic acid B content is 1.2296 mg/g,36.58 mg/;change craft Ⅳ:50%of the ethanol concentration,solvent four times,the extraction time 1.5h,tanshinone Ⅱ A,salvianolic acid B content of 1.5842 mg/g,46.62mg/g;changing the process V:50%of the ethanol concentration,solvent amount,extraction time 1h,tanshinone Ⅱ A,salvianolic acid B content is 1.4788 mg/g,and 42.77mg/g;change craft VI:50%of the ethanol concentration,solvent amount,extraction time 2h,tanshinone Ⅱ A,salvianolic acid B content is 1.294 mg/g,31.03 mg/g;the concentration of ethanol 50%solvent four times,the process conditions of the extraction time 1.5h,Tanshinone Ⅱ A and salvianolic acid B content;50%of the ethanol concentration,solvent 2 times,the process conditions of the extraction time 1 h,tanshinone Ⅱ A with Dan phenol acid B content of the second.2:draw a different process conditions under Compound Salvia film target composition of the rat in vivo concentration-time curve:"Chinese Pharmacopoeia" collection contains compound Salvia tablets as reference preparation,selection of process conditions Ⅳ and process conditions Ⅴ rats drug time curve is drawn.Operation as follows:the reference preparation,the conditions Ⅳ and process conditions Ⅴ extract adjusted to the same concentration,respectively gavage to rats,blood,HPLC method was used to determine the content of the target composition in the plasma,and draw out the process under the conditions of the concentration-time curve.Comparative analysis of the curve when each drug the following results:Tanshinone ⅡA of the three preparations,salvianolic acid B in vivo peak plasma concentration is the same time,the in vivo half-life of the same,and three preparations of tanshinone ⅡA of salvianolic acid B in vivo concentration-time area under the curve AUC:process conditions Ⅳ tanshinone Ⅱ A,salvianolic acid BAUC respectively 152.216370(mg/L)*min,and 1081.152590(mg/L),*min;conditions Ⅴ tanshinone Ⅱ A,Salvianolic acid BAUC respectively to is 130.708694(mg/L)*min,and 949.313110(mg/L)*min;reference preparation of tanshinone Ⅱ A,salvianolic acid BAUC respectively for the 101.970795(mg/L)*min,697.314030(mg/L)*min.3:Different leaching conditions fufangsalvia tablet target composition of the body evaluation:under various process conditions in vitro and in vivo data were analyzed at different process conditions,the target composition in vitro and in vivo fufangsalvia tablet fingerprinting of peak the same time,the drug half-life in vivo.Conditions Ⅳ tanshinone Ⅱ A,salvianolic acid B content of 1.5842 mg/g,,46.62mg/g,;AUC were 152.216370(mg/L)*min,1081.152590(mg/L)*min;C(max),respectively.0.307391 ng/ml,11.648401 ng/ml;conditions Ⅴ tanshinone Ⅱ A,salvianolic acid B content is 1.4788 mg/g and 42.77mg/g;AUC were 130.708694(mg/L)*min,949.313110(mg/L),*min;C(max).270040 ng/ml,10.544204 ng/ml;Pharmacopoeia fufangsalvia tablet tanshinone Ⅱ A,salvianolic acid B content was 1.0446 mg/g,31.78 mg/g,;the AUC 101.970795(mg/L)*min,697.3 14030(mg/L)*min;C(max).260234 ng/ml,7.369968 ng/ml.The results showed that high content of drug in vitro,in vivo the AUC,C(max)were higher,and low levels of drugs in vitro,in vivo the AUC,C(max)were lower,indicating that the in vitro content and absorbed into the body of the target composition of compound Salvia tablets,the maximum absorption of concentration are positively correlated.In summary the following conclusions can be drawn:tanshinone Ⅱ A,salvianolic acid B as the quality control of ingredients of compound Salvia tablets,can reflect the intrinsic quality of the preparations,to achieve its controllability;comparison tanshinone Ⅱ A,salvianolic acid B main reason for the in vivo uptake,process conditions Ⅳ,Ⅴ were higher than the Pharmacopoeia of Compound Salvia Tablets,far as this description of the preparation conditions of the Pharmacopoeia of Compound Salvia Tablets is not the best technology,this outcome optimization of process parameters.