Resting-state FMRI Study Of Amygdala-whole Brain Functional Connectivity After Acute Alcohol Exposure In Healthy Young People And The Effect Of Naloxone

Objective To investigate the changes of functional connectivity（FC）between amygdala and whole brain regions after acute alcohol exposure in healthy young adults.Methods 28 healthy young volunteers（age range:24-34y）were recruited to evaluate the cognitive function with the Mini-mental State Examination（MMSE）,MMSE score≥27.Resting-state fMRI data were collected before and 0.5h after drinking,and blood alcohol concentration（BAC）was measured at 0.5h and 1.0h after drinking respectively.DPARSF software was used to preprocess the resting state data,and the bilateral amygdala was used as the seed point to correlate with the time series of all other voxels in the whole brain.（1）The paired sample t test was used to compare the difference between amygdala-whole brain FC before and after drinking;（2）The subjects after drinking were divided into high and low groups according to BAC.The independent sample t test was used to compare the FC difference between the two groups.Results Finally,23 healthy volunteers（12 males and 11 females）were enrolled after screening.As compared with that before drinking,left amygdala-whole brain FC enhanced areas include bilateral occipital lobe,left middle temporal gyrus,left inferior parietal gyrus and left cerebellum,FC decreased in right declive,right superior frontal gyrus,right fusiform gyrus,right hippocampus and parahippocampal gyrus(P _uncorr<0.005,voxels>20);As compared with that before drinking,right amygdala-whole brain FC enhanced areas include left precentral and postcentral gyrus,right cuneus,FC decreased in left cerebellum,left hippocampus and parahippocampal gyrus,right pallidum,right middle frontal gyrus(P _uncorr<0.005,voxels>20).As compared with the low BAC group,FC of right amygdala-whole brain decreased in left cerebellum posterior lobe,left insula,left superior temporal gyrus,left occipital lobe,right frontal lobe,right thalamus in high BAC group(P _uncorr<0.005,voxels>20);There was no significant difference region in FC of left amygdala-whole brain between high and low BAC groups.Conclusion Acute alcohol exposure might cause FC changes between amygdala and multiple brain regions,which provides imaging evidence for related clinical manifestations after acute alcohol consumption;the right cerebral hemisphere may be more susceptible to alcohol.Objective To explore the effect of naloxone on functional connectivity（FC）between amygdala and whole brain regions in resting-state in healthy young adults after acute alcohol exposure.Methods Participants who completed the experiment I were given the same amount of alcohol again one week later.After drinking for 0.5 h,venous blood was collected to measure blood alcohol concentration（BAC）and 1.0 ml of naloxone was injected intravenously.Then,resting-state functional magnetic resonance data was collected.BAC was measured again after drinking for 1.0 h.DPARSF software was used to preprocess the resting state data,and the bilateral amygdala was used as the seed point to correlate with the time series of all other voxels in the whole brain.The paired sample t test was used to compare the amygdala-whole brain FC changes between naloxone group and non-naloxone group after acute drinking.Results Finally,Twenty-three healthy volunteers（12 males and 11 females）were enrolled after screening.As compared with non-naloxone group,the enhanced regions of left amygdala-whole brain FC after naloxone administration include right cerebellum posterior lobe,left cerebellum inferior semi-lunar lobe,left parahippocampal gyrus,left frontal lobe（orbital part of superior frontal gyrus and inferior frontal gyrust,central anterior gyrus）,left basal ganglia,right frontal lobe（orbital part of middle frontal gyrus,opercular part of inferior frontal gyrus,gyrus rectus）,right paracentral lobule,bilateral insula(P_uncorr<0.005,voxels>20);As compared with non-naloxone group,the enhanced regions of right amygdala-whole brain FC after naloxone administration include right cerebellum,right frontal lobe（inferior frontal gyrus,gyrus rectus）,bilateral inferior temporal gyrus,orbital part of left superior frontal gyrus,left pallidum;FC decreased in bilateral occipital lobe,left superior temporal gyrus and left precentral gyrus(P_uncorr<0.005,voxels>20).Conclusions BAC decreased significantly after using naloxone,and naloxone could reverse the change of FC between amygdala and multiple brain regions caused by acute alcohol,which provided image evidence for clinical efficacy evaluation.