Effect Of Long No-coding RNA Lnc-ALX1-2:10 On Proliferation And Migration Of Prostate Cancer Cells

Background:Prostate cancer(PCa)is the second-highest morbidity and fifth fatality rate among men in the world.The prognosis of patients with early localized prostate cancer has made great progress,but the five-year survival rate of metastatic prostate cancer is still very low.Related studies have shown that prostate cancer is related to the expression and dysfunction of long non-coding RNA(lncRNA),while we still know little about the expression and functional changes of lncRNAs in the process of proliferation and migration of prostate cancer,which needs to be further explored.Objective:To find novel lncRNAs related to the proliferation and migration of prostate cancer cells and to explore its possible mechanism,to provide a new target for the diagnosis and treatment of prostate cancer.Methods:The differentially expressed lncRNA,in high metastatic prostate cancer cell line(PC-3M-1E8)and low metastatic prostate cancer cell line(PC-3M2B4)was analyzed by transcriptome sequencing,and differentially expressed lncRNAs were screened out.The RNA expression level was verified by quantitative real-time PCR.According to the results of qRT-PCR,a small number of LncRNAs were selected for further study.To knock down the selected lncRNAs,we transfect PC-3M-1E8 with correspondent siRNAs and verify the effect on cell proliferation,migration,and EMT-related protein markers.Then the lncRNA with the strongest inhibitory effect was selected for further research.Finally,we compare the differences of mRNAs in PC-3M-1E8 by transcriptome sequencing after Lnc-ALX-2:10 knockdown and explore the possible mechanism using bioinformatics approach.Finally,we verified the alteration of mRNAs expression related to proliferation and migration by qRT-PCR and the markers by Western blot.Results:Compared with PC-3M-2B4,PC-3M-1E8 has up-regulated expression of many lncRNAs,especially the lnc-ALX1-2 cluster.Among them,the knockdown of Lnc-ALX1-2:10 can significantly inhibit the proliferation and migration of PC-3M1E8 cells,which is achieved by reversely regulating a large number of genes related to angiogenesis,proliferation and migration.Conclusion:Lnc-ALX1-2:10 was a powerful regulator for proliferation and migration of PCa cell.The research suggested that lnc-ALX1-2:10 was a potential therapeutic target of PCa and possible biomarker for evaluating PCa metastasis.