Preliminary Studies On The Relationship Between Clinical Features And Gut Microbiota In Children With Henoch-Schonlein Purpura

Objective:This study aimed to explore the relationship between clinical features and gut microbiota in children with Henoch-Schonlein Purpura(HSP).Methods:Fecal and blood samples were collected from 34 children with HSP(including 18 with primary HSP and 16 with recurrent HSP)and 23 healthy children.16SrDNA sequencing was performed to detect gut microbiota,and bioinformatics analysis was performed on the sequencing results.Statistical methods were used to analyze the correlation between the gut microbiota sequencing results and the clinical characteristics of the children(primary and recurrent,children with abdominal HSP and without abdominal).Results:1.Compared with the healthy control group,the α diversity indexes in the primary HSP group was significantly decreased,and the difference between the two groups was statistically significant(P<0.05),and the β diversity indexes shows the significant difference in the microbial community among the primary HSP groups,the recurrent HSP group and healthy control.At the phylum level,the relative abundance of Proteobacteria had an increasing tendency in HSP groups compared with that in healthy control group(P<0.05),At the genus level,the relative abundance of Faecalibacterium and Roseburia were decrease in HSP groups,while a increase in the abundance of the genera Escherichia-Shigella and Fusobacterium in the HSP groups.2.The abundance of Escherichia-Shigella in the recurrent HSP group was significantly higher than that in the primary HSP group,and the ROC curve was constructed with the AUC value of 0.750.3.According to the Spearman correlation analysis,the abundance of Bacteroides was positively associated with the serum IgG level of children with HSP(r=0.5347,P<0.05),while the abundance of Lachnoclostridium was negatively correlated with Complement 3(r=-0.5523,P<0.05).4.The Sobs index,Chao index and Ace index of gut microbiota in HSP group with abdominal were higher than those in HSP group without abdominal and healthy control group.There was no significant difference in Simpson index among the three groups.According to PCOA analysis,gut microbiota in HSP group with abdominal was different from that in the healthy control group and the HSP group without abdominal,which showed that the abundance of Actinobacteria and Proteobacteria in HSP group with abdominal was higher than that in the other two groups,and the abundance of Faecalibacterium in HSP group with abdominal was decreased,while the abundance of Streptococcus and Fusobacteria were increased.Conclusions:1.The gut microbiota of children with HSP was different from that of healthy children.2.The genus Escherichia-Shigella has a diagnostic value for HSP recurrence.3.The Bacteroides and Lachnoclostridium may affect IgG and complement C3 levels in children with HSP.4.The abdominal symptoms of HSP children were related to gut microbiota(Streptococcus and Butyric acid bacteria).