Expression And Clinical Significance Of TLR4 And MyD88 In Oral Squamous Cell Carcinoma

Objective: The goal of this study was to evaluate the expression level of TLR4(toll-like receptor 4)and My D88(myeloid differentiation factor 88)in OSCC(oral squamous cell carcinoma),and to explore its role in the occurrence and development of OSCC,so as to provide help for the diagnosis and treatment of OSCC.Materials and Methods:A total of 55 patients with oral squamous cell carcinoma were collected from the Stomatological Hospital Affiliated to Guangxi Medical University from January 2020 to September 2020,all of whom were pathologically diagnosed.Oral squamous cell carcinoma tissues and adjacent mucosal tissues of each patient were cut,and the protein and m RNA expression levels of TLR4 and My D88 in oral squamous cell carcinoma tissues and adjacent mucosal tissues were detected by immunohistochemistry and real-time quantitative PCR(SYBR Green I).The relationship between the expression levels of the above two factors and the clinical indicators was analyzed.Results :The protein expression of TLR4 and My D88 in oral cancer was higher than that in adjacent mucosal tissues by immunohistochemistry(P <0.001).The protein expression of My D88 in highly to moderately differentiated oral squamous cell carcinoma is higher than that in poorly differentiated oral squamous cell carcinoma(P <0.05).However,there was no significant correlation with age,sex,ethnicity,tumor location,TNM stage,contralateral lymph node metastasis,smoking and alcohol consumption(P>0.05).The results of RT-q PCR showed that the expression of TLR4 m RNA in oral squamous cell carcinoma tissues with different growth time was significantly different(P <0.05).However,there was no significant correlation with age,gender,ethnicity,tumor site,TNM stage,contralateral lymph node metastasis,smoking and alcohol consumption(P > 0.05).Conclusion:The protein overexpression of TLR4 and My D88 in oral squamous cell carcinoma suggests that TLR4 and My D88 play a certain role in the development and progression of oral squamous cell carcinoma,which may be used to determine the differentiation degree and prognosis of oral squamous cell carcinoma,and may provide a new direction for personalized and precise treatment of oral squamous cell carcinoma patients.