| Objective:To explore the effects of early enteral nutrition on the degree of acute gastrointestinal injury,gastrointestinal hormone secretion and IL-17 expression of inflammatory cytokines in brain tissue induced by cerebral infarction in rats,so as to provide theoretical basis for clinic.Methods:32 healthy SD male rats(7-8 weeks old,weight 250g-280g)were randomly divided into 4 groups:normal group(Control group),sham operation group(Sham group),cerebral infarction model group(Model group)and early enteral nutrition treatment group(Treatment group),with 8 rats in each group.Model group and Treatment group were established according to the modified middle cerebral artery embolization method,Sham group only skin incision,nerve separation and other operations,no vascular ligation,normal group did not do treatment.The first three groups were given normal saline,4ml,4 times a day,while the Treatment group was given Baepril nutrient solution with the same dose and frequency as the former three groups.The detection indexes were as follows:(1)the rats in each group were scored by neurobehavioral score 4 hours after modeling by Longa method;(2)the rats in each group were weighed before and 48 hours after modeling by electronic scale,and the weight loss was calculated;(3)the extracted gastrointestinal tissue was stained by HE staining,observed under light microscope and scored for gastrointestinal mucosal injury.(4)the expression of motilin(MTL)and gastrin(GAS)in serum and IL-17 in brain tissue of experimental rats were detected by enzyme linked immunosorbent assay(ELISA);(5)the brain tissue of experimental rats was stained with red tetrazolium(TTC)staining and the size of cerebral infarction was observed.Results:1.The results of neurobehavioral score at 4 hours after modeling in each group showed that the neurobehavioral score in the model group was significantly higher than that in the normal group and sham operation group(P<0.001).There was no significant difference in neurobehavioral score between the treatment group and the model group(P>0.05).2.The results ofweight loss of rats in each group before and 48 hours after modeling showed that:There was no significant difference in body weight before modeling among all groups(P>0.05).48 hours after modeling,the body weight of rats in the model group was significantly lower than that in the normal group,sham operation group and treatment group(P<0.001).These results suggest that early enteral nutrition can significantly improve the weight loss caused by high metabolism after cerebral infarction.3.The results of gastrointestinal injury in each group showed that:(1)gross observation:compared with the normal group and the sham operation group,congestion,erosion,bleeding point and even gastrointestinal bleeding could be seen on the surface of the model group,and congestion and edema could be seen in the treatment group compared with the model group.but bleeding points,erosion and other rare.(2)microscopic observation:compared with the normal group and the sham operation group,defects,villus lodging,disordered arrangement of glands,obvious infiltration of inflammatory cells and severe injury could be seen in the mucosal layer of the model group,compared with the model group,the layers of the treatment group were clearly displayed,the glands and villi of the mucosal layer were disordered,but the overall arrangement was relatively neat,and the infiltration of inflammatory cells was not obvious.(3)The results of histopathological score of gastrointestinal mucosa showed that:The score of model group was significantly higher than that of normal group and sham operation group(P<0.001),and the score of the treatment group also increased significantly(P<0.01).However,compared with the model group,the score of the treatment group decreased(P<0.05).4.The results of ELISA showed that the expressions of GAS,MTL and IL-17 in the model group were significantly higher than those in the normal group and sham operation group(P<0.001),and the treatment group also increased(P<0.01).Compared with the model group,the expression of GAS,MTL and IL-17 in the treatment group decreased(P<0.05).5.The results of gross and TTC staining of brain tissue showed that:(1)gross observation:compared with the normal group and the sham operation group,the cerebral edema on the infarcted side of the model group was significantly increased,and that of the treatment group was also increased,but slightly smaller than that of the model group;(2)TTC staining:because there was no vascular occlusion in the normal group and sham operation group,only brain tissue TTC staining was made in the model group and treatment group.After staining,the infarct size of the treatment group was slightly smaller than that of the model group,suggesting that early entera lnutrition,may reduce the volume of cerebral infarction by reducing intracranial inflammation.Conclusions:1.Early enteral nutrition can reduce the high energy consumption in rats with acute cerebral infarction.2.Early enteral nutrition can reduce the expression of gastrointestinal hormones in serum of rats with cerebral infarction,and may further improve the acute gastrointestinal injury after cerebral infarction by this mechanism.3.Early enteral nutrition can reduce the expression of inflammatory cytokines in the brain tissue of rats with cerebral infarction,which may reduce the volume of cerebral infarction and further improve the prognosis of cerebral infarction. |
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