Study On The Correlation Between Angiotensin Ⅱ,Vascular Endothelial Growth Factor And Arteriosclerosis Obliterans And Its TCM Syndromes

Objective: To explore the correlation between angiotensin Ⅱ(AngⅡ),vascular endothelial growth factor(VEGF)and atherosclerosis obliterans(ASO)and its TCM Syndromes.Methods: In the first study,a total of 60 ASO patients who met the inclusion and exclusion criteria were selected as the observation group,and30 healthy subjects were selected as the control group.The general conditions of the two groups(gender,age,smoking history,diabetes history,hypertension history),arterial pressure(systolic blood pressure and diastolic blood pressure)were collected,and the duration,location,Fontaine stage and ankle brachial index(ABI)of ASO patients were also analyzed.AngⅡ,VEGF,uric acid(UA),low density lipoprotein(LDL),high density lipoprotein(HDL),triglyceride(TG)and total cholesterol(TC)were measured in the two groups.The differences of the above indexes between the two groups were compared,and the levels of AngⅡ and VEGF in patients with ASO were studied according to the general situation,course of disease,location of onset,Fontaine stage and ABI risk level,so as to explore the correlation between AngⅡ,VEGF and ASO.In the second study,a total of 60 ASO patients who met the inclusion and exclusion criteria in study 1 were selected,including 30 cases in the blood stasis type group and 30 cases in the damp-heat injection type group.The correlation between the above indexes and TCM syndrome types was discussed,and the differences of AngⅡ and VEGF between the two groups were compared.Results: Study1:(1)Compared with the control group,the proportion of males,smoking history,diabetes history,and hypertension history in ASO patients was higher(P<0.05);diastolic blood pressure,LDL,TC,AngⅡ,and VEGF levels were higher(P<0.05);HDL level was lower(P<0.01).(2)The level of AngⅡ in men with ASO was significantly higher than that in women(P<0.05);with the increase of age,the levels of AngⅡ and VEGF in ASO patients increased sequentially(P<0.01);the levels of AngⅡ and VEGF in ASO Fontaine stage Ⅱand Ⅲ,stage Ⅳ in the three groups increased sequentially(P<0.01).(3)Logistic regression analysis showed that AngⅡ and VEGF were risk factors for ASO.(4)The area under the ROC curve(AUC)of AngⅡ in the diagnosis of ASO was 0.764 and the Youden index was 0.65,corresponding to a sensitivity of 98.3% and a specificity of 66.7%;the AUC of VEGF in the diagnosis of ASO was 0.854 and the Youden index was 0.666,the corresponding sensitivity was 73.3%,and the specificity was 93.3%;the AUC of AngⅡ and VEGF in the diagnosis of ASO was 0.901,the Youden index was 0.70,the corresponding sensitivity was 86.7%,and the specificity was 83.3%.Study2:Compared with ASO in blood stasis group,the duration of ASO in patients with damp heat type was longer(P<0.01),smoking history,diabetes history and history of hypertension were higher(P<0.05),HDL level and ABI were lower(P<0.05),AngⅡ and VEGF levels were higher(P<0.05).Conclusion:(1)AngⅡ and VEGF are correlated with the occurrence and development of ASO;the two have certain reference value for the diagnosis of ASO;(2)The expression levels of AngⅡ and VEGF are different in ASO damp-heat injection group and blood stasis type group,can be used as a useful supplement to the objectification of ASO TCM syndrome differentiation.