Effect Of Qigong Wan Combined With Antagonist Regimen On VEGF And IL-6 In Obese PCOS Patients With Phlegm-dampness Evidence Of IVF

Objective:To investigate the effects of Qigong Wan on the clinical efficacy,improvement of Chinese clinical symptoms and signs,and the incidence of ovarian hyperstimulation syndrome（OHSS）in obese polycystic ovary syndrome（PCOS）patients undergoing IVF-ET through the intervention of Qigong Wan in patients with phlegm-damp evidence in in vitro fertilization and embryo transfer（IVF）.We investigated the effectiveness of Qigong Wan on the clinical efficacy,improvement of clinical symptoms and signs in Chinese medicine and its effect on the incidence of ovarian hyperstimulation syndrome（OHSS）in patients with PCOS in IVF-ET.This study provides a safe and effective treatment for obese PCOS patients with phlegm-dampness evidence of IVF.Methods:Sixty patients with obese polycystic ovary syndrome（PCOS）with phlegm-dampness evidence undergoing IVF-ET were selected and divided into observation group（Qigong Wan+western medicine treatment）and control group（western medicine treatment）according to random number table.30 patients in each group were treated with gonadotropin-releasing hormone（Gn RH）antagonist regimen to promote ovulation.In the observation group,the first day of Gn RH injection was combined with Qigong Wan,while in the control group,there was no Chinese medicine intervention.Basal endocrine levels and basal sinus follicle count（AFC）were recorded,body mass index（BMI）was calculated,and phlegm-dampness symptoms were scored in both groups.The levels of luteinizing hormone（LH）,estradiol（E₂）,progesterone（P）,number of eggs obtained,fertilization rate,quality embryo rate,incidence of OHSS,and clinical pregnancy rate were compared.The correlation between OHSS and serum and follicular fluid vascular endothelial growth factor（VEGF）and interleukin-6（IL-6）levels was analyzed.Results:1.After treatment,the observation group had significantly lower phlegm and dampness symptom scores than the control group,and the difference was statistically significant（P<0.05）.2.The levels of LH on HCG day in the observation group were lower than those in the control group,and the levels of E₂were higher than those in the control group,and the differences were statistically significant（P<0.05）.3.The number of eggs obtained in the observation group was higher than that in the control group,and the difference was statistically significant（P<0.05）.4.The probability of OHSS in the observation group was significantly lower than that in the control group,and the difference was statistically significant（P<0.05）.6.The serum IL-6 level on HCG day was reduced in the observation group compared with the control group,and the difference was statistically significant（P<0.05）.7.The follicular fluid level of VEGF was reduced in the observation group compared with the control group,and the difference was statistically significant（P<0.05）.VEGF levels were reduced in the observation group compared with the control group,and the difference was statistically significant（P<0.05）.8.The correlation analysis between the occurrence of OHSS and follicular fluid VEGF levels,serum and follicular fluid IL-6 levels showed no statistically significant differences（P>0.05）;the correlation analysis between serum VEGF levels and the occurrence of OHSS showed statistically significant differences（P<0.05）.Conclusion:1.The use of Qigong wan combined with antagonist regimen in the treatment of obese PCOS patients with phlegm-dampness evidence in IVF can effectively improve the clinical symptoms of phlegm-dampness evidence in PCOS patients,reduce the number of Gn days,increase the serum E2 level on HCG day,the number of eggs obtained,the fertilization rate,the rate of high-quality embryos,and reduce the follicular fluid VEGF and serum IL-6levels.2.Serum VEGF level correlated with the occurrence of OHSS.Follicular fluid VEGF levels and serum and follicular fluid IL-6 levels did not correlate with the occurrence of OHSS.