Correlation Between Atopic Dermatitis And Intestinal Flora In Infancy(0-6 Months)

Objective:1.Distribution and abundance of intestinal microflora in children with atopic dermatitis(0-6 months)and healthy infants;2.Compare the severity of atopic dermatitis in children with different feeding methods;3.To compare the quantity and richness of intestinal flora in healthy infants under different feeding methods;so as to lay a theoretical foundation for further clinical application of intestinal microbiot-related treatment methods for the prevention and treatment of children with atopic dermatitis.Methods:1.Case collection: 19 AD infants were enrolled in the dermatology department of Suining Central Hospital,all of whom met the criteria set by Williams in 1994 with no gender limitation.According to different feeding methods,they were divided into breast-fed AD1 group(12 cases)and formula fed AD2 group(7 cases).Twenty-seven healthy controls without allergic diseases and without taking antibiotics in the past one month were included.According to different feeding methods,they were divided into breast-feeding HC1 group(n = 15)and formula-feeding HC2 group(n = 12).All selected subjects were approved by the Ethics Committee of Suining Central Hospital of Sichuan Province.Stool samples from AD infants and healthy controls were collected and stored at-80℃.2.Specimen detection: All collected stool samples were sent to the High-throughput sequencing laboratory of Norhe Zhiyuan Technology Co.,Ltd.in Tianjin for intestinal flora detection.3.Data analysis: SPSS19.0 software was used to analyze the statistical difference of Alpha diversity between groups by Wilcoxon rank sum test.P < 0.05 indicated that the difference was statistically significant.Results:1.Baseline of subjects: 19 AD infants and 27 healthy controls included in this study were permanent residents in Sichuan Province,and there was no statistical significance in gender and age between the two groups(P> 0.05).2.Severity of disease: The severity of AD infants included in this study was mainly moderate,with a SCORAD score of(50±10)3.Intestinal flora distribution:At the phylum level,there was a significant increase in Firmicutes in the AD2 group compared with the healthy control group.Proteobacteria,Fusobacteria,Bacteroidetes and Actinobacteriota were not significantly different.At class level,only Clostridium increased significantly.At the target level,Clostridiales were significantly increased in AD2 group,and Bifidobacteriales were significantly increased in AD1 and HC1 groups compared with AD2 and HC2 groups.At the family level,the number of Clostridiaceae in AD2 group was significantly higher than that in the other three groups,and the number of Bifidobacteriaceae in AD1 and HC1 group was significantly higher than that in AD2 and HC2 group.At the genus level,the number of Clostridium in AD2 group is significantly higher than that in the other three groups,and the number of Bifidobacterium in AD1 and HC1 group is significantly higher than that in AD2 group.But there was no significant difference between the two groups(P> 0.05).Conclusion:1.The intestinal microflora of infants(0-6 months)with atopic dermatitis differs from that of healthy infants of the same age.2.In the intestinal flora of infants fed by formula milk powder,the abundance of dominant bacteria such as bifidobacteria decreased,and the abundance level of intestinal flora of infants fed by formula milk powder was lower than that of infants fed by formula milk powder.3.The occurrence of atopic dermatitis in infancy(0-6 months)may be related to the increased abundance of Clostridium flora;The increase in clostridium may be related to formula feeding.