The Effect Of Long Non-coding RNA LINC01123 On Biological Characteristics Of Cervical Squamous Cell Carcinoma And The Preliminary Study On Its Mechanism

Objective: To investigate the expression of LINC01123 in cervical cancer,and to explore the effect of LINC01123 on the sensitivity of cisplatin to cervical cancer proliferation and migration.Methods:(1)The expression of LINC01123 in cervical cancer was analyzed by GEPIA database.Cervical cancer tissue and paracancer tissue samples were collected from our hospital from June 2021 to February 2022,and the expression of LINC01123 in cervical cancer tissue was verified by lnc RNAs microarray analysis and q RT-PCR.(2)The basic information and clinical data of patients were summarized,and the relationship between expression level and clinical characteristics was analyzed.(3)ROC curve was constructed to evaluate the diagnostic value of LINC01123 in cervical cancer.(4)The expression of LINC01123 in human normal cervical epithelial cell line Hcer Epic、cervical squamous cell line Si Ha and C33 A was determined by q RT-PCR.(5)The effects of LINC01123 on Si Ha cell proliferation,migration,and cisplatin sensitivity were investigated by lentiviral knockdown of LINC01123 levels in Si Ha cells.(6)Adenovirus overexpressed LINC01123,and the effect of overexpression on the proliferation of Si Ha cells was observed.(7)Western blot and q RT-PCR were used to detect SLC7A11 and GPX4 expression in Si Ha cells after LINC01123 knockdown.(8)The effect of LINC01123 on the transcription level of Bcl-2 and ZEB1 in Si Ha cells was detected by q RT-PCR.Results:(1)GEPIA predicted the high expression of LINC01123 in cervical cancer.lnc RNAs chip sequencing and q RT-PCR results showed that LINC01123 was highly expressed in cervical cancer tissues compared with corresponding adjacent tissues,and the difference was statistically significant(P<0.05).(2)Cervical cancer tissue and paracancer tissue specimens were collected from 27 patients,including 24 patients with squamous cell carcinoma,22.2% of whom were younger than 45 years old.FIGO stage of the patients was A1 to A1 According to the expression level of LINC01123,patients were divided into the high expression group and the low expression group.Combined with clinical data,the two groups were compared.The results showed that there was no statistically significant difference in clinical variables such as age stage infiltration depth between the two group(P>0.05).(3)he sensitivity of LINC01123 in the diagnosis of cervical cancer was81.2%,and the AUC of ROC curve was 0.732(P<0.01).LINC01123 was more valuable in diagnosis than CEA and SCC.(4)The expression of LINC01123 in cervical squamous cell line Si Ha and C33 A was significantly higher than that in normal cervical epithelial cell line Hcer Epic((P<0.01)),and Si Ha expression was the highest.(5)LINC01123 in Si Ha cells was knocked down,and the results of CCK8 cell scratches showed that cell proliferation was significantly inhibited in sh-LINC01123 group(P<0.05);CCK8 measured that THE IC20 of cisplatin on Si Ha cells was about 5 μg/m L.At this concentration,the inhibition rate of sh-LINC01123 cells was 31.57 ± 3.58% and Si Ha was 21.39 ± 2.13%,the difference was statistically significant(P<0.05).(6)Overexpression of LINC01123 in Si Ha cells significantly promoted cell proliferation compared with the control group(P<0.05).(7)q RT-PCR and Western blot results showed that LINC01123 knockdown inhibited the transcription and expression of SLC7A11 and GPX4,and the difference was statistically significant compared with the control group(P<0.05).(8)LINC01123knockdown inhibited the transcription of anti-apoptotic protein Bcl-2 EMT-related protein ZEB1,while overexpression showed the opposite result,with statistically significant differences in transcription levels(P<0.01).Conclusion:(1)LINC01123 is highly expressed in cervical cancer tissues and cervical squamous cell carcinoma,and is expected to become a new biomarker in the diagnosis of cervical cancer.(2)LINC01123 promotes proliferation,migration and drug resistance of cervical squamous cell Si Ha cells.(3)LINC01123 may regulate the transcription and expression of SLC7A11 and GPX4,and the transcription of Bcl-2 and ZEB1,thus playing a role in promoting cancer,which may provide a new target for the treatment of cervical cancer.