Preparation Of Transparent Plant Membrane And Its Loading Of Adipose Stem Cells To Promote Wound Healing

Wound is a common clinical problem,and the speed and quality of healing in wound healing is gradually becoming more important.Improperly treated wound infections add to the patient’s burden,especially when chronic infections occur,resulting in a dramatic decrease in the speed and quality of healing.At present,clinical wound treatment options are mainly based on a variety of wound dressings,with the main functions focused on promoting healing and antibacterial,which play a certain therapeutic role.However,it is worth noting that all the wound dressings currently in clinical use are non-transparent dressings,which makes it possible to observe the infection and healing of the wound only during dressing changes.Topical or systemic antibiotics are one of the main tools for the prevention and treatment of wound infections,but the associated resistance and toxicity problems have limited their use in clinical practice.In addition,studies have shown that early local or systemic antibiotics have a destructive effect on the growth factors and active substances that promote healing of the wound,so we urgently need to develop a dressing that can detect infection early and treat it quickly with non-antibiotic means,that is an integrated diagnosis and treatment at the early stage of infection.In this paper,we used the edible Allium fistulosum endothelial membrane(AFEM)as the main body and its special physical structure to load the adipose stem cells(ADSCs)with promoting wound healing.AFEM was applied to rat wounds and the wounds were monitored in real time,and it was found that it had a significant effect on wound healing.In addition,due to the good penetration performance of AFEM,the antimicrobial blue light can directly and effectively pass through the transparent membrane to treat the wounds as needed.The antimicrobial treatment was performed as needed.In conclusion,this system can detect infection at an early stage and provide antimicrobial treatment for wounds in cooperation with blue light,thus achieving early detection of infection,timely and effective treatment of infection,and promoting wound healing.In vitro and in vivo experiments have demonstrated that this dressing system has the functions of easy monitoring of wounds,early detection of infection and treatment,and promotion of wound healing,which provides a new idea for the development of a more suitable clinical wound dressing.