| Objective: To observe Jiaji electroacupuncture combined with treadmill training on behavioral score,toluidine blue staining sciatic nerve myelinated axon count and HGF/c-Met expression in SD rats after sciatic nerve transection injury,and to explore Jiaji electroacupuncture combined with running.The mechanism of the axon regeneration of peripheral nerve injury promoted by Taiwanese training provides a theoretical basis for the treatment of sciatic nerve injury with Jiaji electroacupuncture combined with treadmill training,and for the repair of peripheral nerve injury.Methods: A total of 180 SD male rats were randomly coded and divided into 5 groups:normal group,model group,treadmill treatment group,Jiaji electroacupuncture group,Jiaji electroacupuncture combined with treadmill group,36 rats in each group.Then they were divided into 3 subgroups according to 1 week,2 weeks and 4 weeks after treatment,with 12 animals in each subgroup.Sunderland Ⅴ degree injury model was induced on the left hind limb sciatic nerve of SD rats by suture after amputation.The normal group did not do any intervention,and the model group was placed in a rat cage after modeling and fed normally.Before the formal training,the treadmill group underwent adaptive training for 3 days,and then started formal training after 3 days of successful modeling.After the formal training,the slope of the treadmill is set to 0°,the speed of the treadmill is set to 8m/min for 1 to 3days,12m/min for 4 to 7 days,and gradually adjusted to 15m/min after 7 days of training,and the basic training is from Monday to Saturday.The training time was 6 days per week,with rest on Sunday,and each training session was 30 minutes;the Jiaji electro-acupuncture group was given Huatuo Jiaji acupuncture and combined electro-acupuncture treatment intervention,once a day,20 times a day.Minutes/time;Jiaji electroacupuncture combined with treadmill training group,after successful modeling,first treadmill training,and then Jiaji electroacupuncture treatment.After 1 week,2 weeks,and 4 weeks of treatment in each group,the samples were collected at the corresponding intervention time points,and the sciatic nerve function index was calculated by the footprint method for behavioral scoring.Toluidine blue staining was used to count the myelinated axons of the sciatic nerve.Expression of HGF and c-Met protein in spinal cord and sciatic nerve.Results:1.Sciatica Motor Function Index(SFI)After 1 week,2 weeks and 4 weeks of treatment intervention,the SFI value of rats at each time point increased gradually with time,and there was a significant difference in general(P< 0.05).The SFI scores of SD rats in the acupuncture group,treadmill group,combination group and model group were all lower than those in the normal group,and the difference was statistically significant(P<0.05).The SFI scores of rats in the combined group were higher than those in the model group,and the difference was statistically significant(P<0.05).Significant(P<0.05);there was no significant difference in SFI score between Jiaji EA group and treadmill group(P>0.05);after 2 weeks of treatment intervention,the SFI score of SD rats in normal group was higher than that in Jiaji EA group The difference was statistically significant(P<0.05);among them,the SFI scores of the rats in the model group were lower than those in the Jiaji electroacupuncture group,the treadmill group,and the combined group,the SD was greater There was a statistically significant difference in the SFI score of mice(P<0.05);The SFI score of SD rats in the combined group was significantly higher than that in the two simple treatment groups(jiaji electroacupuncture group and treadmill group),and the difference was statistically significant(P<0.05);rats in Jiaji electroacupuncture group and treadmill group There was no significant difference in SFI score(P>0.05);after 4 weeks of treatment intervention,the SFI scores of SD rats in the combination group were higher than those in the model group,Jiaji electroacupuncture group and treadmill treatment group,and the difference was statistically significant(P<0.05).0.05);among them,the SFI scores of the rats in the Jiaji EA group and the treadmill treatment group were lower than those in the combination group,and the difference was statistically significant(P<0.05);and the SD rats in the Jiaji EA group and the treadmill treatment group were There was no significant difference in the SFI scores of SD rats(P>0.05);except for the normal group,the SFI scores of SD rats in each group reached the peak at the 4-week time point.2.Toluidine Blue Myelinated Axon CountAfter 1 week,2 weeks and 4 weeks of treatment intervention,the axonal regeneration of the sciatic nerve in the three treatment groups was higher than that of the model group at the same time point,and lower than that of the normal group,and the difference was statistically significant(P< 0.05);after the treatment intervention for 1 week,the axonal regeneration of SD rats in the combination group was significantly higher than that in the Jiaji electroacupuncture group and the treadmill treatment group,and the difference was statistically significant(P<0.05);There was no significant difference in axon regeneration between the electroacupuncture group and treadmill treatment group(P>0.05);after 2 weeks of treatment intervention,the axon regeneration of SD rats in the combined group was higher than that in the two simple treatment groups(electroacupuncture group).Acupuncture group,treadmill group)and model group,the difference was statistically significant(P<0.05);there was no significant difference in axon regeneration between Jiaji electroacupuncture group and treadmill treatment group(P>0.05);treatment intervention After 4 weeks,the axonal regeneration of SD rats in the combination group was higher than that in the model group and the two simple treatment groups,and the difference was statistically significant(P<0.05);the axonal regeneration of SD rats in the model group was lower than that in the treatment group.There was no significant difference in the regeneration of axons between the Jiaji electroacupuncture group and the treadmill treatment group(P>0.05).Myelinated axon counts all peaked at the 4-week time point.3.Immunohistochemical detection of HGF expression in sciatic nerve and spinal cord Spinal cord segment: After 1 week,2 weeks,and 4 weeks of treatment intervention,the expression of HGF in spinal cord of rats in Jiaji electroacupuncture group,treadmill treatment group,and combination group was higher than that in the model group at the same time point.There was statistical significance(P<0.05);and the expression of HGF in the spinal cord of the rats in the combination group at each time point was better than that in the simple electroacupuncture group and the treadmill group,and the difference was statistically significant(P<0.05);treatment intervention 1 After 1 week and 2 weeks,there was no significant difference in the expression of HGF in the spinal cord between the Jiaji EA group and the treadmill treatment group(P>0.05).The expression of HGF in the spinal cord of the rats in the group was lower than that in the combination group,and the difference was statistically significant(P<0.05).There was no significant difference(P>0.05);the expression of HGF in the spinal cord of SD rats in each group increased at 1 week and reached a peak at 2 weeks.Sciatic nerve: After treatment for 1 week,2 weeks and 4 weeks,the expression of HGF in the sciatic nerve of SD rats in Jiaji electroacupuncture group,treadmill group and combination group was higher than that in the model control group,and the differences were statistically significant.and the expression of HGF in the sciatic nerve of SD rats in the combined group was significantly higher than that in the model group and the two simple treatment groups(jiaji electroacupuncture group and treadmill treatment group)at the same time point,and the difference was statistically significant After 4 weeks of treatment intervention,the expression of HGF in the sciatic nerve of SD rats in the combination group was higher than that in the treadmill group and Jiaji electroacupuncture group,and the difference was statistically significant(P<0.05).);there was no significant difference in the expression of HGF in the sciatic nerve of SD rats between the treadmill group and Jiaji electroacupuncture group(P>0.05).(Jiaji electroacupuncture group and treadmill treatment group)the expression of HGF in the sciatic nerve of SD rats was higher than that of the model group,and the difference was statistically significant(P<0.05);the expression of HGF in the sciatic nerve of SD rats in each group The amount increased at 1 week and reached a peak at 2 weeks.4.Immunohistochemical detection of c-Met expression in sciatic nerve and spinal cord Spinal cord segment: After 1 week,2 weeks,and 4 weeks of treatment intervention,intraspinal c-Met of SD rats in 2 simple treatment groups(jiaji electroacupuncture group,treadmill treatment group)and combined treatment group The expression level of SD rats in the combined group was higher than that in the model control group at the same time point,and the difference was statistically significant(P<0.05).In the treadmill group,the difference was statistically significant(P<0.05);after 1 week and 2 weeks of treatment intervention,the spinal cord of SD rats in the two simple treatment groups(jiaji electroacupuncture group and treadmill treatment group)There was no significant difference in the expression of c-Met(P>0.05);after 4 weeks of treatment intervention,the intraspinal c-Met of SD rats in two simple treatment groups(jiaji electroacupuncture group and treadmill treatment group)The expression of c-Met in the spinal cord of SD rats in the two simple treatment groups(jiaji electroacupuncture group and treadmill treatment group)had no There was a significant difference(P>0.05);the expression of c-Met in the spinal cord of SD rats in 5 groups after treatment intervention reached a peak at 7-14 days,and returned to normal at 4 weeks.Sciatica: The expression of c-Met in the sciatic nerve of SD rats in the combination group and 2 simple treatment groups(jiaji electroacupuncture group and treadmill treatment group)after three time points of treatment intervention for 1 week,2 weeks and 4 weeks Compared with the model control group,the difference in expression was statistically significant(P<0.05);and the expression level of c-Met in the sciatic nerve of SD rats in the combined group was better than that in the normal group and the two simple treatment groups(Jiaji)at the same time point.The expression of c-Met in the sciatic nerve of SD rats in the combination group was higher than that in the treadmill group and the treadmill group after 4weeks of treatment intervention.Jiaji electroacupuncture group,the difference was statistically significant(P<0.05);there was no significant difference in the expression of c-Met in the sciatic nerve of SD rats between the treadmill group and Jiaji electroacupuncture group(P>0.05).After 1 week and 4 weeks,there was no significant difference in the expression of c-Met in the sciatic nerve of SD rats in the two simple treatment groups(jiaji electroacupuncture group and treadmill treatment group)(P>0.05);SD was greater in each group.The expression of c-Met in the sciatic nerve of mice reached a peak at 7-14 days,and the highest at 2 weeks.In conclusion:(1)Jiaji electroacupuncture,treadmill training,Jiaji electroacupuncture combined with treadmill training can improve the SFI score and sciatic nerve myelinated axon count after sciatic nerve dissection and injury,and promote HGF/c-Met in the sciatic nerve and spinal cord.expression,and the combined group was better than the simple Jiaji EA group and the treadmill group.(2)Jiaji electroacupuncture combined with treadmill training promotes nerve regeneration,which may be related to the up-regulation of HGF/c-Met expression in the sciatic nerve and spinal cord,which may be one of the factors that promote the recovery of peripheral nerves after injury.As a neurotrophic factor and angiogenesis factor,HGF may be involved in nerve protection and tissue repair and regeneration after sciatic nerve amputation. |
PDF Full Text Request