Study On Mechanism Of Based On PI3K/AKT Pathway To Explore The Mechanism Of Xiaoshen Formula In Diabetic Kidney Disease

Objective:The study aims to establish a diabetic kidney disease model and explore the mechanism of self-designed herbal compound Xiaoshen Formula based on PI3K/AKT pathway to improve diabetic kidney disease under the guidance of Jiedu Tongluo Baoshen Method.To provide theoretical basis for clinical treatment of diabetic kidney disease.Methods:83 healthy male SD rats were collected from 200 to 220g.After 5 days of adaptive feeding in SPF animal experimental center,12 normal SD rats were randomly selected as blank control group.After fasting for 72h,then STZ solution 60 mg·kg^-1was intraperitoneally injected into the left lower abdomen to prepare the diabetic animal model.2 weeks later,the rats were injected with STZ solution again.The successfully induced diabetic rats were randomly divided into model group,losartan potassium control group,Xiaoshen Formula high-dose,medium-dose and low-dose groups.After 11 weeks of continuous intragastric treatment,urine was collected for 24h.Compared with the normal group,urine protein in the model group was significantly higher,which was regarded as the successful preparation of diabetic kidney disease rat model.At the same time,body weight,blood glucose,renal function level and renal histopathological changes of rats in each group were detected,and the expression levels of inflammatory factors and protein were detected by ELSIA method,so as to evaluate the therapeutic effect of treatment group on DKD rats.Results:1 The rats in the model group showed emaciation,increased blood glucose,changes in renal function and obvious pathological changes of renal tissue under light microscope,which proved that the animal model of diabetic nephropathy was successfully established.2 body weight:Compared with model group,the high-dose xiaoshen Formula group significantly improved the emaciation symptom of diabetic kidney disease rats（P<0.01）.3 Blood glucose:Compared with model group,serum GLU in treatment group was significantly decreased（P<0.05）.4 Renal function:Compared with model group,serum UA and serum CRE in Xiaoshen Formula high-dose group were significantly decreased（P<0.01）;Serum BUN and urine Ucr were significantly increased（P<0.05）.5 Pathological results:Compared with the model group,the renal status of rats in the high-dose xiaoshen Formula group was significantly improved,and only a small amount of renal tubular epithelial cells had watery degeneration.6 Protein content of PI3K and p-AKT:Compared with model group,the expressions of PI3K and p-AKT in Xiaoshen Formula high-dose group were significantly decreased（P<0.01）.7 serum cytokine expression:Compared with model group,the expressions of fibrogenic growth factor TGF-β1 and pro-inflammatory factors IL-1,IL-6,TNF-αand MCP-1 in serum of xiaoshen Formula high-dose group were significantly decreased（P<0.01）.Conclusion:1 Under the guidance of Jiedu Tongluo Baoshen method,Xiaoshen Formula can effectively improve the general state,body weight,blood glucose,renal function（BUN,Ucr,UA,CRE）and pathological state of kidney tissue under light microscope of diabetic kidney disease rats,so as to delay the development of diabetic kidney disease.2 Xiaoshen Formula reduces the expression of PI3K,p-AKT protein and related key inflammatory expressions（TGF-β1,TNF-α,MCP-1,IL-1,IL-6）.It is preliminarily concluded that Xiaoshen Formula inhibits inflammatory response by regulating PI3K/AKT signaling pathway.Play a role in the treatment of diabetic kidney disease.3 This study proves that Jiedu Tongluo Baoshen method is an effective method for the treatment of diabetic kidney disease,which provides experimental basis and theoretical basis for its clinical application.