| ObjectivePersistent periapical periodontitis is mostly caused by long-term bacterial infection in the root ca nal.Enterococcus faecalis(E.faecalis)is a common bacteria in persistent periapical periodontitis.Therefore,the effective removal of E.faecalis is of great significance for the treatment of persistent apical periodontitis.Root canal therapy is currently the conventional method for the treatment of persistent apical periodontitis.Most of the infected microorganisms can be removed through instrum ent preparation.However,due to the complexity of the anatomical structure of the root canal system,some narrow areas cannot be reached by instrument preparation,resulting in microorganisms remaining in the root canal.In the root canal system,the smea r layer is formed on the root canal wall during instrument preparation,which will also affect the removal of microorganisms and root canal filling.To some extent,chemical irrigation can remove contaminants and smear layer from areas that are not accessi ble to instrument preparation.At present,the commonly used root canal irrigation s in clinic have certain advantages but also have certain deficiencies.To analyze the effect of herbal antibacterial solution on E.faecalis infection in root canal,herbal antibacterial solution was compared with 3% sodium hypochlorite,3% hydrogen peroxide,and normal saline in this experiment.The results provide a basis for the effective removal of E.faecalis from the root canal by herbal antibacterial solution,and provi de a reference for the discovery of a root canal irrigation with more convenient clinical operation and wider application scope.MethodsAccording to strict inclusion and exclusion criteria,cattle foreteeth that met the inclusion criteria were selected to be included in the experiment.The roots were cut,the standard working length of the root canal was set to 20 mm,and the roots wereprepared to 25# with K file.The apical foramen was sealed with fluid resin,and all the teeth were sterilized by high pressure steam.E.f a e c a l i s(ATCC29212)was inoculated into the root canal and cultured for 28 days to establish an infected root canal model.The samples of teeth were randomly divided into 4 groups with 10 samples in each group.The groups were as follows: group A(herbal antibacterial solution),group B(3% sodium hypochlorite),group C(3% hydrogen peroxide),and group D(normal saline).Each group of samples wa s mechanically prepared with EDTA gel and irrigated with a different irrigation solution after each preparation.Samples were prepared by instruments and different chemical irrigations.Eight samples from each group were selected to calculate the changes in the number of E.faecalis in the root canals after irrigation.The remaining 2 samples were subjected to scanning electron microscopy to observe the degree of dentin tubul e opening and smear layer cleaning.SPSS 26 was used for corresponding statistical analysis to compare the bactericidal effect of the herbal antibacterial solution and the other three irrigations on E.faecalis in infected root canals.ResultsThe bacterial culture count results showed that the bacterial amount after root canal irrigation in groups A,B,C,and D were significantly lower than that in the infected root canals(P<0.001).There was no significant difference in bacterial count between group A an d group B(P>0.05).There were significant differences in bacterial counts between group A and groups C or D(P<0.05).The smear layer removal effect of groups A,B,C,and D all showed that the root crown 1/3 > the middle 1/3 > the apical 1/3.The removal ability of smear layer in the same area between group A and B were comparable,and both were better than groups C and D.ConclusionsThe results of this experiment show that the herbal antibacterial solution and 3% Na OCl have the same effect on the removal of E.faecalis in the infected root canal.And with the combined use of EDTA,the two have the same effect on the removal of the smear layer in the root canal,which provid e reference for clinical use of herbal antibacterial solution as root canal irrigation. |
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