Study On VKORC1 Gene Polymorphism And Risk Factors In Patients With Coronary Heart Disease In Xining Area

Objective:VKORC1 gene polymorphisms affect warfarin dose-response and vitamin K cycling by altering VKORC1 enzymatic activity,leading to abnormal coagulation,while playing a role in bone mineralization,arterial calcification,and arteriosclerosis.Thrombosis and arteriosclerosis can directly lead to vascular diseases such as coronary heart disease and stroke.There are different clinical studies on the relationship between VKORC1 gene and CHD at home and abroad.The purpose of this study was to explore the correlation between VKORC1+2255T/C and susceptibility to acute myocardial infarction in patients with acute myocardial infarction in Xining area,in order to provide some theoretical support for screening high-risk groups of acute myocardial infarction.Methods:101 patients with acute myocardial infarction who met the inclusion and exclusion criteria were collected as the acute myocardial infarction group and 118 patients with non-coronary heart disease as the control group;blood samples from all patients were collected,and the sangers method was used to determine the VKORC1 genotype by DNA sequencing;acute myocardial infarction was analyzed.The general data,laboratory test,genotype and allele distribution of the infarction group and the control group were used to determine the correlation between VKORCl gene polymorphism and acute myocardial infarction susceptibility by binary logistic regression.Results:This study found that the distribution of VKORC1+2255T/C genotype and allele between acute myocardial infarction group and control group was significantly different(P<0.05),but CC genotype was not found.In order to avoid the interference of potential confounding factors,binary logistic regression was used to correct age,sex,smoking history,hypertension history,and diabetes history.95%CI 1.112-7.212,P=0.029).The stratified analysis of VKORC1+2255T/C by sex showed that the frequency of TC genotype and C allele at VKORC1+2255 locus in the male acute myocardial infarction group was significantly higher than that in the control group(P<0.05).In order to avoid the interference of potential confounding factors,after adjusting for age and diabetes history by binary logistic regression,it was found that the association between TC genotype at VKORC1+2255 and acute myocardial infarction still existed(OR=3.085,95%CI 1.010-9.427,P=0.048).There was no significant difference in the distribution of VKORC1+2255T/C genotype and allele between the acute myocardial infarction group and the control group in females(P>0.05).Potential confounding such as age,history of hypertension,and history of diabetes were corrected by binary logistic regression.After the factor,this correlation still did not exist(OR=3.668,95%CI 0.508-26.467,P=0.197).Conclusions:VKORC1+2255T/C genotype and allele are associated with susceptibility to acute myocardial infarction.TC genotype may be a significant genetic risk factor for acute myocardial infarction in Xining,and this risk is more obvious in males.This study provides a new perspective for exploring the role of VKORC1 gene polymorphisms in the pathogenesis of acute myocardial infarction.