A Study Of Chemokine (C-X-C Motif) Ligand 1 Protein/CXC Receptor 2 Participate In Brain White Matter Injury In Neonatal Rats Via The Gut-brain Axis

Objective:Explore the pathogenesis of brain white matter injury in premature infants through the study of whether Chemokine(C-X-C motif)Ligand 1 Protein(CXCL1)and CXC Receptor 2(CXCR2)as inflammatory mediator participat in brain white matter injury in neonatal rats via the gut-brain axis.Methods:Sixty-nine neonatal SD rats were randomly divided into five groups:normal group,necrotizing enterocolitis(NEC)group,sham surgery group,hypoxic-ischemic brain injury(HIBI)group,and NEC+HIBI group.The neonatal SD rats were gived gavage with 3%dextran sodium sulfate to construct the NEC model,and 3-day-old SD rats were treated with hypoxic ischemia to construct the HIBI model.HE staining observe the changes of brain and intestinal pathology in neonatal rats.Western blot technic to check the expression of CXCL1、CXCR2 in brain and intestinal tissue of neonatal rats.Result:Compared to the normal rats,The periventricular white matter appeared pathological injury in the NEC group,Which was more severe in the NEC+HIBI group.Moreover,inflammation and necrosis occurred in intestinal tissue of the HIBIgroups neonatal rats.Westem blot technic indication:Compared to the normal group,The expression levels of CXCL1 and CXCR2 increased at different degrees in the intestinal and brain tissues of the NEC group、HIBI group and NEC+HIBI group neonatal rats.Compared to the HIBI group,The expression of CXCL1 and CXCR2 continuously increased in intestinal and brain tissues of the NEC+HIBI group neonatal rats(P<0.05).Conclusion:CXCL1/CXCR2 may transmit intestinal inflammatory factors via the gut-brain axis and participated in brain white matter injury in neonatal rats.